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Titolo:
Dynamics of adhesive rupture between fibroblasts and fibronectin: microplate manipulations and deterministic model
Autore:
Thoumine, O; Meister, JJ;
Indirizzi:
Swiss Fed Inst Technol, Biomed Engn Lab, CH-1015 Lausanne, Switzerland Swiss Fed Inst Technol Lausanne Switzerland CH-1015 ausanne, Switzerland
Titolo Testata:
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
fascicolo: 6, volume: 29, anno: 2000,
pagine: 409 - 419
SICI:
0175-7571(2000)29:6<409:DOARBF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION; MECHANICAL-PROPERTIES; LOCOMOTING CELLS; INITIAL BINDING; DETACHMENT; KINETICS; STRENGTH; FORCES; FLOW; MEMBRANES;
Keywords:
micromanipulation; ligand-receptor interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Meister, JJ Swiss Fed Inst Technol, Biomed Engn Lab, CH-1015 Lausanne, Switzerland Swiss Fed Inst Technol Lausanne Switzerland CH-1015 tzerland
Citazione:
O. Thoumine e J.J. Meister, "Dynamics of adhesive rupture between fibroblasts and fibronectin: microplate manipulations and deterministic model", EUR BIOPHYS, 29(6), 2000, pp. 409-419

Abstract

To characterize the dynamics of cell-substrate adhesive rupture, we used anovel micromanipulation technique, in which individual fibroblasts seized on a rigid microplate were placed into contact with a fibronectin-coated flexible microplate, then pulled away. The fibronectin density (0-3000 molecules/mu m(2)) and the pulling rate (1-10 mu m/s) were controlled. The extentof the contact zone decreased to zero at a time threshold corresponding toadhesive rupture. The uniaxial force at the interface, computed from the deflection of the microplate, increased linearly with time and reached a maximum before dropping to zero. A deterministic model, focusing on the mean number of bonds between fibronectin and its membrane receptor on the cell surface, shows rapid rupture when the force reaches a critical value, in agreement with experimental observations. Increasing the ligand density and therate of load raises the maximal force (30-200 nN), in reasonably good agreement with the model predictions. Minimization of error between experimental and simulated forces allowed identification of two physicochemical properties of the bond, i.e. its association rate constant (k(on)(2D) = 3 x 10(-4) mu m(2)/s) and structural length (d = 3 nm). These results may help understand better fibroblast locomotion and interaction with the extracellular matrix.

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Documento generato il 03/06/20 alle ore 00:01:08