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Titolo:
CYTOKINE RNA EXPRESSION IN AN EQUINE CD4-KDA SURFACE PROTEIN( SUBSET DIFFERENTIATED BY EXPRESSION OF A NOVEL 46)
Autore:
BYRNE KM; DAVIS WC; HOLMES MA; BRASSFIELD AL; MCGUIRE TC;
Indirizzi:
WASHINGTON STATE UNIV,COLL AGR & HOME ECON,DEPT ANIM SCI PULLMAN WA 99164 WASHINGTON STATE UNIV,COLL VET MED,DEPT VET MICROBIOL & PATHOL PULLMAN WA 99164 UNIV CAMBRIDGE,DEPT CLIN VET MED CAMBRIDGE CB3 0ES ENGLAND
Titolo Testata:
Veterinary immunology and immunopathology
fascicolo: 3-4, volume: 56, anno: 1997,
pagine: 191 - 204
SICI:
0165-2427(1997)56:3-4<191:CREIAE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LYMPHOCYTES-T; MOLECULAR-CLONING; CELL ACTIVATION; MONOCLONAL-ANTIBODIES; INTERLEUKIN-2; CD38; CDNA;
Keywords:
EQUINE; CYTOKINES; T-LYMPHOCYTE; SUBSET; WC4; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Byrne et al., "CYTOKINE RNA EXPRESSION IN AN EQUINE CD4-KDA SURFACE PROTEIN( SUBSET DIFFERENTIATED BY EXPRESSION OF A NOVEL 46)", Veterinary immunology and immunopathology, 56(3-4), 1997, pp. 191-204

Abstract

Two monoclonal antibodies (MAb), HB65A (IgG2a) and HB86A (IgGI), recognize a unique cell surface molecule on equine T-lymphocytes. The molecule, designated EqWC4, identified by these MAbs is present on a subpopulation of CD4 + equine lymphocytes (6.3-10.2% of Arabian lymphocytesCD4 + WC4 +) and a smaller population of CD8 + lymphocytes (0.5% to 1.2% of Arabian lymphocytes CD8 + WC4 +). EqWC4 is absent from B-lymphocytes, granulocytes, and macrophages. Both MAbs bound to a 46-kDa protein following immunoprecipitation reactions with lysates of surface labeled thymocytes. Immunoaffinity purification using HB65A yielded two molecules of 46 kDa and 52 kDa under reducing conditions and a third 92-kDa molecule was present in nonreduced conditions. Activation by mitogen did not increase expression of EqWC4 on equine lymphocytes. Lymphocytes from Arabian, Pony, and Thoroughbred breeds showed a common distribution of EqWC4 among leukocytes. However, there were significantlyfewer Pony lymphocytes bound to HB65A and HB86A when compared to Arabian and Thoroughbred breeds. Using reverse transcriptase-polymerase chain reaction (RT-PCR), magnetically enriched populations (to 80% of cells isolated) of EqWC4 + lymphocytes expressed a cytokine RNA profile dominated by -interleukin2 (IL-2) and interferon-gamma (IFN-gamma) forunstimulated cells. Upon mitogen stimulation, IL-4 was also expressedat low levels while the IL-2 levels decreased and the IFN-gamma levels increased relative to unstimulated cells. EqWC4 is similar to CD28 in molecular weight and its formation of dimers and could therefore be the equine orthologue. However, because of the differences in CD28 expression, EqWC4 probably represents a previously uncharacterized equinelymphocyte marker. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:31:17