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Titolo:
A liquid chromatography-mass spectrometry method for the quantification ofbioavailability and bioconversion of beta-carotene to retinol in humans
Autore:
Wang, Y; Xu, XY; van Lieshout, M; West, CE; Lugtenburg, J; Verhoeven, MA; Creemers, AFL; van Breemen, RB;
Indirizzi:
Univ Illinois, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 & Pharmacognosy, Chicago, IL 60612 USA Univ Wageningen & Res Ctr, Div Human Nutr & Epidemiol, Wageningen, Netherlands Univ Wageningen & Res Ctr Wageningen Netherlands ageningen, Netherlands Leiden Univ, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands Leiden UnivLeiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands Nutr Res & Dev Ctr, Bogor 16112, Indonesia Nutr Res & Dev Ctr Bogor Indonesia 16112 Dev Ctr, Bogor 16112, Indonesia
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 20, volume: 72, anno: 2000,
pagine: 4999 - 5003
SICI:
0003-2700(20001015)72:20<4999:ALCSMF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRESSURE CHEMICAL-IONIZATION; METABOLISM; VEGETABLES; PREVENTION; DISEASE; MEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: van Breemen, RB Univ Illinois, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Chicago, IL 60612 USA
Citazione:
Y. Wang et al., "A liquid chromatography-mass spectrometry method for the quantification ofbioavailability and bioconversion of beta-carotene to retinol in humans", ANALYT CHEM, 72(20), 2000, pp. 4999-5003

Abstract

A method based on high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (APCI LC-MS) was developed for the quantification of the bioavailability of retinyl palmitate and beta-carotene and the bioconversion of beta-carotene to retinol in humans. Following oral administration of [8,9,10,11,12,13,14,15,19,20-C-13(10)]-retinyl palmitate and [12,13,14,15,20,12',13',14',15',20'-C-13(10)]-beta-carotene at physiological doses to children between 8 and 11 years of age, blood samples were drawn and serum was prepared. Retinol and beta-carotene were extracted from 0.2- and 1.0-mL serum samples, respectively, and analyzed using reversed-phase HPLC with a C-30 column interfaced to an APCI mass spectrometer. Unlike other LC-MS assays for carotenoids, no additional purification steps were necessary, nor was any derivatization of retinol or beta-carotene required. APCI LC-MS showed a linear detector response for beta-carotene over 4orders of magnitude. Using selected ion monitoring to record the elution profile of protonated circulating beta-carotene at m/z 537 and [C-13(10)]-beta-carotene at m/z 547, the limit of detection was determined to be 0.5 pmol injected on-column. To assess the ratio of labeled to unlabeled retinol, selected ion monitoring was carried out at m/z 269, 274, and 279, These abundant fragment ions corresponded to the loss of water from the protonated molecule of circulating retinol, [C-13(5)]-retinol (metabolically formed from orally administered [C-13(10)]-beta-carotene), and [C-13(10)]-retinol (formed by hydrolysis of [C-13(10)]-retinyl palmitate); The ratios of labeled to unlabeled retinol and the ratio of labeled to unlabeled beta-carotene were calculated. Combined with standard HPLC measurement of beta-carotene andretinol concentration and a mathematical model, these results showed that this simple LC-MS method can be used to quantify beta-carotene bioavailability and its bioconversion to retinol at physiologically relevant doses.

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Documento generato il 04/04/20 alle ore 08:28:16