Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Integrated nuclear fluorescence and expression of hormone-binding sites inmalignant pleural effusions
Autore:
Kayser, K; Markle, C; Kugler, C; Andre, S; Schuring, MP; Zeng, FY; Gabius, HJ;
Indirizzi:
Thoraxklin, Dept Pathol, D-69126 Heidelberg, Germany Thoraxklin Heidelberg Germany D-69126 athol, D-69126 Heidelberg, Germany Thoraxklin, Dept Thorac Surg, D-69126 Heidelberg, Germany Thoraxklin Heidelberg Germany D-69126 Surg, D-69126 Heidelberg, Germany Univ Munich, Inst Physiol Chem, D-8000 Munich, Germany Univ Munich Munich Germany D-8000 t Physiol Chem, D-8000 Munich, Germany Thoraxklin, Dept Thorac Surg, Heidelberg, Germany Thoraxklin Heidelberg Germany in, Dept Thorac Surg, Heidelberg, Germany
Titolo Testata:
ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
fascicolo: 5, volume: 22, anno: 2000,
pagine: 364 - 372
SICI:
0884-6812(200010)22:5<364:INFAEO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
STATIC DNA CYTOMETRY; HUMAN-BREAST-CANCER; HUMAN-LUNG CANCER; LECTIN HISTOCHEMISTRY; STEROID-RECEPTORS; DIAGNOSTIC-AID; CELL; IDENTIFICATION; PROTEIN; GLYCOLIGANDS;
Keywords:
pleural effusion, malignant; fluorescence; integrated nuclear fluorescence; hormone-binding sites;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kayser, K Thoraxklin, Dept Pathol, Amalienstr 5, D-69126 Heidelberg, Germany Thoraxklin Amalienstr 5 Heidelberg Germany D-69126 erg, Germany
Citazione:
K. Kayser et al., "Integrated nuclear fluorescence and expression of hormone-binding sites inmalignant pleural effusions", ANAL QUAN C, 22(5), 2000, pp. 364-372

Abstract

OBJECTIVE: To investigate potential disease- and prognosis-associated nuclear and cellular features from cell properties in a prospective study on malignant pleural effusions. STUDY DESIGN: Integrated nuclear fluorescence and the expression of binding capacities of carrier-immobilized estradiol, progesterone and testosterone; and of labeled sarcolectin; and the presence of calcyclin were measured in 50 cases with proven malignant pleural effusions (10 rnesotheliomns, 40 metastasizing tumors). A double fluorescence technique using the fluorochrome DAPI and a Texas Red-based avidin-biotin detection system were applied. Detailed clinical data, including the follow-up for up to 40 months, were included. RESULTS: Pleural effusions in all patients with mesotheliomas occurred prior to (9/10) or at the time of histologic confirmation. Mesotheliomas had the highest tumor cell fraction (12.4%) in S phase and breast carcinomas thelowest (10.7%). More than 80% of malignant cells expressed binding capacities for the applied probes. A statistically significant correlation was noted between the S-phase-related turner cell friction and the expression of progesterone receptors. Survival was associated with tumor origin, treatmentby pleurodesis, and certain cytometric and histochemical features. CONCLUSION: The immunofluorescence double-staining technique can be applied successfully in malignant effusions to combine DNA measurements with those of immunohistochemical and ligand histochemical reactivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 18:52:49