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Titolo:
N-hydroxysuccinimidyl fluorescein-O-acetate as a highly fluorescent derivatizing reagent for aliphatic amines in liquid chromatography
Autore:
Wang, H; Li, J; Liu, X; Zhang, HS;
Indirizzi:
Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China Wuhan Univ Wuhan Peoples R China 430072 m, Wuhan 430072, Peoples R China
Titolo Testata:
ANALYTICA CHIMICA ACTA
fascicolo: 1, volume: 423, anno: 2000,
pagine: 77 - 83
SICI:
0003-2670(20001020)423:1<77:NFAAHF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRECOLUMN DERIVATIZATION; SECONDARY-AMINES; GAS-CHROMATOGRAPHY; CHLORIDE; AIR; DANSYLATION; POLYAMINES; SAMPLES; URINE;
Keywords:
N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA); aliphatic amines; liquid chromatography (LC); fluorescence detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, HS Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China Wuhan UnivWuhan Peoples R China 430072 30072, Peoples R China
Citazione:
H. Wang et al., "N-hydroxysuccinimidyl fluorescein-O-acetate as a highly fluorescent derivatizing reagent for aliphatic amines in liquid chromatography", ANALYT CHIM, 423(1), 2000, pp. 77-83

Abstract

A sensitive fluorescent reagent, N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA), has been developed as a precolumn derivatizing reagent for aliphatic amines in liquid chromatography (LC). SIFA was shown to be a successful reagent with advantages including high fluorescent quantum yield in the visible region, specificity to aliphatic amines, mild derivatization conditions and few by-products. Methylamine, ethylamine, ethylene diamine, isopropylamine, isobutylamine, n-butylamine, 1,4-butylene diamine and 1,5-amylene diamine were used to investigate the separation and derivatization conditions. In a pH 8.5 H3BO3-Na2BO7 buffer solution, SIFA reacted with amines at 45 degrees C for 30 min to form stable derivatives. The derivatives could beseparated on a C-18 column in 18 min with methanol-water (46/54, v/v) containing 10 mmol l(-1) pH 5.40 citric acid-Na2HPO4 buffer as a mobile phase. The detection limits of these amines were in the range 85-520 amol (signal-to-noise ratio = 3), which were better than those using the other derivatizing reagents for amine analysis by LC. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 05/04/20 alle ore 03:50:00