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Titolo:
Induction of telomerase activity in fibroblasts from bleomycin-injured lungs
Autore:
Nozaki, Y; Liu, TJ; Hatano, K; Gharaee-Kermani, M; Phan, SH;
Indirizzi:
Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Dept Pathol, Ann Arbor, MI 48109 USA
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 4, volume: 23, anno: 2000,
pagine: 460 - 465
SICI:
1044-1549(200010)23:4<460:IOTAIF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PULMONARY FIBROSIS; MUSCLE ACTIN EXPRESSION; IMMORTAL CELLS; CONTRACTILE PHENOTYPE; DIFFERENTIATION; SEQUENCE; TISSUES; CANCER; RAT; (TTAGGG)N;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Phan, SH Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA UnivMichigan Ann Arbor MI USA 48109 ol, Ann Arbor, MI 48109 USA
Citazione:
Y. Nozaki et al., "Induction of telomerase activity in fibroblasts from bleomycin-injured lungs", AM J RESP C, 23(4), 2000, pp. 460-465

Abstract

Bleomycin-induced lung injury causes increased fibroblast numbers in the lung and pulmonary fibrosis. Studies of fibroblasts isolated from such injured lungs have revealed evidence of increased intrinsic proliferative capacity, but the mechanism is unknown. Telomerase catalyzes the addition of telomeric DNA repeats onto chromosomal ends, which is associated with increasedcellular life span or immortality. To examine whether telomerase might play a role in regulating fibroblast proliferative capacity in pulmonary fibrosis, lung fibroblasts were isolated from rats treated with endotracheal injections of phosphate-buffered saline or bleomycin. At selected time points,the rats were killed and lung fibroblasts isolated. The isolated cells andlung tissue were then used in experiments for measurement of telomerase activity. The results show undetectable telomerase activity in fibroblasts isolated from control uninjured lungs, or in the control lung tissue extracts. Similar results were obtained in cells and lung tissue from Days 1, 3, and 28 bleomycin-injured lungs. However, significant telomerase activity was detected in fibroblasts and tissue extracts isolated from Days 7, 14, and 21 bleomycin-treated rat lungs, with maximal activity observed in the Day 14samples. Analysis of the isolated cells for telomerase messenger RNA or reverse transcriptase expression, combined with alpha-smooth-muscle actin expression by immunohistochemistry, revealed that telomerase expression localized primarily to nonmyofibroblasts. These findings suggest that in additionto elevated growth factor expression, the injured lung fibroblast population may contain cells with increased life span, which could contribute to the observed overall increase in lung fibroblast numbers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:41:00