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Titolo:
Sperm washing and virus nucleic acid detection to reduce HIV and hepatitisC virus transmission in serodiscordant couples wishing to have children
Autore:
Pasquier, C; Daudin, M; Righi, L; Berges, L; Thauvin, L; Berrebi, A; Massip, P; Puel, J; Bujan, L; Izopet, J;
Indirizzi:
Hop Purpan, Virol Lab, Toulouse, France Hop Purpan Toulouse FranceHop Purpan, Virol Lab, Toulouse, France CHU La Grave, Ctr Sterilite Masculine, CECOS Midi Pyrenees, Toulouse, France CHU La Grave Toulouse France ine, CECOS Midi Pyrenees, Toulouse, France CHU La Grave, Human Fertil Res Grp, Toulouse, France CHU La Grave Toulouse France ve, Human Fertil Res Grp, Toulouse, France Hop Lagrave, Dept Obstet, Toulouse, France Hop Lagrave Toulouse FranceHop Lagrave, Dept Obstet, Toulouse, France Hop Purpan, Dept Infect Dis, Toulouse, France Hop Purpan Toulouse France op Purpan, Dept Infect Dis, Toulouse, France
Titolo Testata:
AIDS
fascicolo: 14, volume: 14, anno: 2000,
pagine: 2093 - 2099
SICI:
0269-9370(20000929)14:14<2093:SWAVNA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; SEXUAL TRANSMISSION; NEGATIVE WOMEN; SEMINAL FLUID; HCV RNA; SEMEN; MEN; INSEMINATION; SPERMATOZOA; ABSENCE;
Keywords:
HIV RNA; hepatitis C virus; seminal plasma; seminal cells; spermatozoa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Pasquier, C Hop Purpan, Virol Lab, Toulouse, France Hop Purpan Toulouse France pan, Virol Lab, Toulouse, France
Citazione:
C. Pasquier et al., "Sperm washing and virus nucleic acid detection to reduce HIV and hepatitisC virus transmission in serodiscordant couples wishing to have children", AIDS, 14(14), 2000, pp. 2093-2099

Abstract

Background: Use of a motile spermatozoa isolation process was assessed forreducing the transmission of HIV and hepatitis C virus (HCV) during artificial insemination in HIV-serodiscordant couples in which the man is infected. Patients: Thirty-two HIV-l-infected clinically asymptomatic men, having a median CD4 cell count of 396 x 10(6)/1 and a median blood plasma HIV-1 RNA content of 414 copies/ml. Of these, 16 were infected with both HIV and HCV. Methods: Motile spermatozoa were isolated from 51 semen samples by densitygradient and 'swim-up'. HIV-1 and HCV genomes were detected and quantifiedin the blood plasma and seminal plasma, and detected in seminal cell fractions obtained during spermatozoa isolation. Results: HIV-1 RNA was detected in 30% of seminal plasma samples. HIV-1 genomes were found in 18% of seminal cell samples, but in none of the motile spermatozoa fractions after 'swim-up'. There was no correlation between theHIV-1 RNA concentrations in the blood and seminal plasma. HIV-1 genome wasdetected intermittently in patients who gave more than one sample. HCV RNAwas detected in 20% of seminal plasma samples from HCV viraemic patients, but in no seminal cells or motile spermatozoa fractions. Conclusions: Purification of motile spermatozoa by density gradient plus 'swim-up' reduced the HIV-1 and HCV genomes in the semen of infected individuals to undetectable levels. This method, associated with a standardized virus assay, could be useful for serodiscordant couples (males infected) who wish to have children. (C) 2000 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:32:46