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Titolo:
Expression of the ETA and ETB endothelin receptor subtype mRNA in human detrusor cultured smooth muscle cells
Autore:
Obara, K; Walden, PD; Hatano, A; Tomita, Y; Takahashi, K; Lepor, H;
Indirizzi:
Niigata Univ, Sch Med, Dept Urol, Niigata 9518510, Japan Niigata Univ Niigata Japan 9518510 ed, Dept Urol, Niigata 9518510, Japan
Titolo Testata:
UROLOGIA INTERNATIONALIS
fascicolo: 2, volume: 65, anno: 2000,
pagine: 68 - 72
SICI:
0042-1138(2000)65:2<68:EOTEAE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN URINARY-BLADDER; VASOCONSTRICTOR PEPTIDE; FUNCTIONAL EXPRESSION; HUMAN PROSTATE; CLONING; CDNA; LOCALIZATION; FAMILY; ET(A); RNA;
Keywords:
endothelin receptor; detrusor cultured smooth muscle cells; human; RT-PCR; in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Obara, K Niigata Univ, Sch Med, Dept Urol, Asahimachi 1, Niigata 9518510, Japan Niigata Univ Asahimachi 1 Niigata Japan 9518510 a 9518510, Japan
Citazione:
K. Obara et al., "Expression of the ETA and ETB endothelin receptor subtype mRNA in human detrusor cultured smooth muscle cells", UROL INTERN, 65(2), 2000, pp. 68-72

Abstract

The aim of this study was to determine the expression of the endothelin receptor subtype mRNAs in human detrusor cultured smooth muscle cells using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH), First strand cDNA was made from human detrusor cultured smooth muscle cells total RNA and used for PCR with primers designed to amplify fragments of the ETA and ETB endothelin receptor subtype cDNA sequences. Subcloned fragments of the ETA and ETB endothelin receptor cDNAs were used tosynthesize digoxigenin-labeled cRNA probes by in vitro transcription, COS-7 cells transfected with the ETA and ETB receptor cDNAs were used as positive control and to confirm the absence of cross-hybridization due to sequence homology, Both ETA and ETB receptor mRNAs were detected by RT-PCR analysis. By ISH, both ETA and ETB receptor subtype mRNAs were detected. However, ETA signal was much more intense than ETB signal. These results indicate that mRNAs for both ETA and ETB receptors are expressed in detrusor smooth muscle cells of human urinary bladder. The ETA receptor is the predominant detrusor ET receptor. Copyright (C) 2000 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:40:00