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Titolo:
Developmental and tissue-specific expression of AHR1, AHR2, and ARNT2 in dioxin-sensitive and -resistant populations of the marine fish Fundulus heteroclitus
Autore:
Powell, WH; Bright, R; Bello, SM; Hahn, ME;
Indirizzi:
Woods Hole Oceanog Inst, Dept Biochem, Woods Hole, MA 02543 USA Woods HoleOceanog Inst Woods Hole MA USA 02543 Woods Hole, MA 02543 USA Haverford Coll, Haverford, PA 19041 USA Haverford Coll Haverford PA USA 19041 rford Coll, Haverford, PA 19041 USA
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 2, volume: 57, anno: 2000,
pagine: 229 - 239
SICI:
1096-6080(200010)57:2<229:DATEOA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARYL-HYDROCARBON-RECEPTOR; NUCLEAR TRANSLOCATOR ARNT; ZEBRAFISH DANIO-RERIO; TROUT ONCORHYNCHUS-MYKISS; CLOSE SEQUENCE SIMILARITY; LIFE-STAGE DEVELOPMENT; DIBENZO-P-DIOXIN; BIPHENYL CONGENERS; LAKE TROUT; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN TCDD;
Keywords:
AHR; ARNT; dioxins; PCBs; fish; Fundulus heteroclitus; resistance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Hahn, ME Woods Hole Oceanog Inst, Dept Biochem, MS 32, Woods Hole, MA 02543 USA Woods Hole Oceanog Inst MS 32 Woods Hole MA USA 02543 A 02543 USA
Citazione:
W.H. Powell et al., "Developmental and tissue-specific expression of AHR1, AHR2, and ARNT2 in dioxin-sensitive and -resistant populations of the marine fish Fundulus heteroclitus", TOXICOL SCI, 57(2), 2000, pp. 229-239

Abstract

Fundulus heteroclitus is a well-characterized marine fish model for studying aryl hydrocarbon toxicity. The F. heteroclitus population in New BedfordHarbor (NBH), a Superfund site in southeastern Massachusetts, exhibits heritable resistance to the toxic effects of planar halogenated aromatic hydrocarbons (PHAHs), including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TC DD) and polychlorinated biphenyls (PCBs). To investigate the role of the aryl hydrocarbon receptor (AHR) signal transduction pathway in PHAH resistance, we measured the relative levels of AHR1, AHR2, and ARNT2 mRNA in whole embryos at different developmental stages and in dissected tissues of adults, comparing expression of these genes in NBH fish with fish from a reference site (Scorton Creek, MA [SC]). Expression of both AHR1 and AHR2 mRNA increased during development, achieving maximum levels Drier to hatching. Maximal embryonic expression of AHR1 was delayed relative to AHR2. Whole NBH and SC embryos exhibited no discernable differences in expression of these genes. As we have previously observed, adult SC fish expressed AHR2 and ARNT2 mRNA in all tissues examined, while AHR1 was expressed predominantly in brain, heart, and gonads. In contrast, AHR1 mRNA was widely expressed in NBH fish, appearing with unusual abundance in gill, gut, kidney, liver, and spleen. ThisAHR1 expression pattern was not observed in the lab-reared progeny of NBH fish, demonstrating that constitutive AHR1 expression in gill, gut, kidney,liver, and spleen is not a heritable phenotype. Furthermore, widespread AHR1 expression was not induced in reference-site fish by TCDD or PCB mixtures, suggesting that aberrant AHR1 expression is not simply a normal physiological response of contaminant exposure. These results identify ubiquitous AHR1 expression as an attribute unique to feral NBH F. heteroclitus, and they represent a first step in determining the regulatory mechanisms underlying this expression pattern and its possible role in TCDD resistance.

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Documento generato il 03/04/20 alle ore 14:09:11