Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Activation of in vivo- and in vitro-derived porcine oocytes by using multiple electrical pulses
Autore:
Grupen, CG; Verma, PJ; Du, ZT; McIlfatrick, SM; Ashman, RJ; Nottle, MB;
Indirizzi:
BresaGen Ltd, Reprod Biol Unit, Adelaide, SA 5000, Australia BresaGen LtdAdelaide SA Australia 5000 nit, Adelaide, SA 5000, Australia
Titolo Testata:
REPRODUCTION FERTILITY AND DEVELOPMENT
fascicolo: 7-8, volume: 11, anno: 1999,
pagine: 457 - 462
SICI:
1031-3613(1999)11:7-8<457:AOIVAI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR TRANSFER; FETAL FIBROBLASTS; IN-VITRO; PARTHENOGENETIC DEVELOPMENT; CELL LINE; EMBRYOS; ADULT; FERTILIZATION; STIMULATION; BLASTOMERES;
Keywords:
blastocyst; in vitro maturation; parthenogenetic development; pig;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Grupen, CG BresaGen Ltd, Reprod Biol Unit, POB 259,Rundle Mall, Adelaide, SA 5000, Australia BresaGen Ltd POB 259,Rundle Mall Adelaide SA Australia 5000 lia
Citazione:
C.G. Grupen et al., "Activation of in vivo- and in vitro-derived porcine oocytes by using multiple electrical pulses", REPROD FERT, 11(7-8), 1999, pp. 457-462

Abstract

The current protocols used to activate pig nuclear transfer embryos are less efficient than those used for other species. To address this problem, the effect of multiple sets of electrical pulses on the parthenogenetic development of in vivo- and in vitro-derived porcine oocytes was examined. Each set of pulses consisted of two 1.5 kV cm(-1) DC pulses of 60 mu s duration each, administered 1 s apart. For in vivo-derived oocytes, application of asecond set of pulses 30 min after the first set increased the proportion of oocytes that developed to the blastocyst stage compared with a single treatment (51 v. 34%). Application of a third set of pulses 30 min after the second set reduced the rate of blastocyst formation compared with two sets of pulses. Tn contrast, the rate of blastocyst formation was greater with one set of pulses compared with two sets for in vitro matured oocytes (31 v. 16%). Additional sets of electrical pulses did not affect the number of cells in blastocysts obtained from either group of oocytes compared with a single treatment. In summary the study demonstrates that the application of a second set of activating pulses 30 min after the first set is beneficial toin vivo-derived oocytes, but detrimental to in vitro matured oocytes, in terms of their ability to develop parthenogenetically to the blastocyst stage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:31:10