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Titolo:
Phenotypic instability and rapid gene silencing in newly formed arabidopsis allotetraploids
Autore:
Comai, L; Tyagi, AP; Winter, K; Holmes-Davis, R; Reynolds, SH; Stevens, Y; Byers, B;
Indirizzi:
Univ Washington, Dept Bot, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ton, Dept Bot, Seattle, WA 98195 USA Univ Washington, Dept Genet, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 n, Dept Genet, Seattle, WA 98195 USA
Titolo Testata:
PLANT CELL
fascicolo: 9, volume: 12, anno: 2000,
pagine: 1551 - 1567
SICI:
1040-4651(200009)12:9<1551:PIARGS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPETITIVE DNA; POLYPLOID EVOLUTION; THALIANA; WHEAT; CHROMOSOMES; SEQUENCES; RNA; MAP; EXPRESSION; SELECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Comai, L Univ Washington, Dept Bot, Box 355325, Seattle, WA 98195 USA UnivWashington Box 355325 Seattle WA USA 98195 tle, WA 98195 USA
Citazione:
L. Comai et al., "Phenotypic instability and rapid gene silencing in newly formed arabidopsis allotetraploids", PL CELL, 12(9), 2000, pp. 1551-1567

Abstract

Allopolyploid hybridization serves as a major pathway for plant evolution,but in its early stages it is associated with phenotypic and genomic instabilities that are poorly understood. We have investigated allopolyploidization between Arabidopsis thaliana (2n = 2x = 10; n, gametic chromosome number; x, haploid chromosome number) and Cardaminopsis arenosa (2n = 4x = 32). The variable phenotype of the allotetraploids could not be explained by cytological abnormalities. However, we found suppression of 20 of the 700 genes examined by amplified fragment length polymorphism of cDNA. Independent reverse transcription-polymerase chain reaction analyses of 10 of these 20 genes confirmed silencing in three of them, suggesting that similar to 0.4% of the genes in the allotetraploids are silenced. These three silenced genes were characterized. One, called K7, is repeated and similar to transposons. Another is RAP2.1, a member of the large APETALA2 (AP2) gene family, andhas a repeated element upstream of its 5' end. The last, L6, is an unknowngene close to ALCOHOL DEHYDROGENASE on chromosome 1. CNG DNA methylation of K7 was less in the allotetraploids than in the parents, and the element varied in copy number. That K7 could be reactivated suggests epigenetic regulation. L6 was methylated in the C. arenosa genome. The present evidence that gene silencing accompanies allopolyploidization opens new avenues to this area of research.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:03:00