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Titolo:
Adhesion induced expression of the serine/threonine kinase Fnk in human macrophages
Autore:
Holtrich, U; Wolf, G; Yuan, JP; Bereiter-Hahn, J; Karn, T; Weiler, M; Kauselmann, G; Rehli, M; Andreesen, R; Kaufmann, M; Kuhl, D; Strebhardt, K;
Indirizzi:
Univ Frankfurt, Dept Obstet & Gynecol, D-60590 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60590 col, D-60590 Frankfurt, Germany Univ Frankfurt, Dept Biol, D-60439 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60439 iol, D-60439 Frankfurt, Germany Univ Hamburg, Zentrum Mol Neurobiol, ZMNH, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 iol, ZMNH, D-20246 Hamburg, Germany Univ Regensburg, Dept Hematol & Oncol, D-93042 Regensburg, Germany Univ Regensburg Regensburg Germany D-93042 , D-93042 Regensburg, Germany
Titolo Testata:
ONCOGENE
fascicolo: 42, volume: 19, anno: 2000,
pagine: 4832 - 4839
SICI:
0950-9232(20001005)19:42<4832:AIEOTS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLO-LIKE KINASES; PROTEIN-KINASE; DROSOPHILA-POLO; SIGNAL-TRANSDUCTION; BINDING PROTEIN; GENE; IDENTIFICATION; CLONING; SPINDLE; CELLS;
Keywords:
polo-like kinase; adhesion; macrophages; serine/threonine kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Strebhardt, K Univ Frankfurt, Dept Obstet & Gynecol, Theodor Stern Kai 7, D-60590 Frankfurt, Germany Univ Frankfurt Theodor Stern Kai 7 Frankfurt Germany D-60590
Citazione:
U. Holtrich et al., "Adhesion induced expression of the serine/threonine kinase Fnk in human macrophages", ONCOGENE, 19(42), 2000, pp. 4832-4839

Abstract

Members of the polo subfamily of protein kinases play crucial roles in cell proliferation. To study the function of this family in more detail,,ve isolated the cDNA of human Fnk (FGF-inducible kinase) which codes for a serine/threonine kinase of 646 aa, Despite the homology to the proliferation-associated polo-like kinase (Plk), tissue distribution of Fnk transcripts and expression kinetics differed clearly, In contrast to Pile no correlation between cell proliferation and Fnk gene expression was found. Instead high levels of Fnk mRNA were detectable in blood cells undergoing adhesion. The transition of monocytes from peripheral blood to matrix bound macrophages wasaccompanied by increasing levels of Fnk with time in culture. Neither treatment of monocytes with inducers of differentiation nor withdrawal of serumdid influence Fnk mRNA levels significantly, suggesting that cell attachment triggers the onset of Fnk gene transcription, The idea that Fnk is part of the signalling network controlling cellular adhesion was supported by the analysis of the cytoplasmic distribution of the Fnk protein and the influence of its overexpression on the cellular architecture. Fnk as fusion protein with GFP localized at the cellular membrane in COS cells. Dysregulated Fnk gene expression disrupted the cellular f-actin network and induced a spherical morphology. Furthermore, Fnk binds to the Ca2+/integrin-binding protein Cib in two-hybrid-analyses and co-immunoprecipitation in assays. Moreover, both proteins were shown to co-localize in mammalian cells, The homology of Cib with calmodulin and with calcineurin B suggests that Cib might bea regulatory subunit of polo-like kinases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 23:05:11