Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Functional sites of interaction between release factor RF1 and the ribosome
Autore:
Wilson, KS; Ito, K; Noller, HF; Nakamura, Y;
Indirizzi:
Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA Univ Calif Santa Cruz Santa Cruz CA USA 95064 A, Santa Cruz, CA 95064 USA Univ Tokyo, Inst Med Sci, Dept Tumor Biol, Minato Ku, Tokyo 1088639, JapanUniv Tokyo Tokyo Japan 1088639 mor Biol, Minato Ku, Tokyo 1088639, Japan
Titolo Testata:
NATURE STRUCTURAL BIOLOGY
fascicolo: 10, volume: 7, anno: 2000,
pagine: 866 - 870
SICI:
1072-8368(200010)7:10<866:FSOIBR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFER-RNA HYDROLYSIS; ELONGATION-FACTOR-G; CRYSTAL-STRUCTURE; PEPTIDYL TRANSFERASE; ANGSTROM-RESOLUTION; AMINO-ACID; EF-TU; MUTATIONS; PROTEIN; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Wilson, KS Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, SantaCruz, CA 95064 USA Univ Calif Santa Cruz Santa Cruz CA USA 95064 uz, CA 95064 USA
Citazione:
K.S. Wilson et al., "Functional sites of interaction between release factor RF1 and the ribosome", NAT ST BIOL, 7(10), 2000, pp. 866-870

Abstract

Translational release factors decipher stop codons in mRNA and activate hydrolysis of peptidyl-tRNA in the ribosome during translation termination. The mechanisms of these fundamental processes are unknown. Here we have mapped the interaction of bacterial release factor RFI with the ribosome by directed hydroxyl radical probing. These experiments identified conserved domains of RFI that interact with the decoding site of the 30S ribosomal subunit and the peptidyl transferase site of the 50S ribosomal subunit. RFI interacts with a binding pocket formed between the ribosomal subunits that is also the interaction surface of elongation factor EF-G and aminoacyl-tRNA bound to the A site. These results provide a basis for understanding the mechanism of stop codon recognition coupled to hydrolysis of peptidyl-tRNA, mediated by a protein release factor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 08:49:47