Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential expression of glycoside residues in the mammalian zona pellucida
Autore:
Aviles, M; Okinaga, T; Shur, BD; Ballesta, J;
Indirizzi:
Univ Murcia, Sch Med, Dept Cell Biol, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 , Dept Cell Biol, E-30071 Murcia, Spain Emory Univ, Sch Med, Dept Cell Biol, Atlanta, GA USA Emory Univ Atlanta GA USA Univ, Sch Med, Dept Cell Biol, Atlanta, GA USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 3, volume: 57, anno: 2000,
pagine: 296 - 308
SICI:
1040-452X(200011)57:3<296:DEOGRI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED CARBOHYDRATE CHAINS; SPERM RECEPTOR ACTIVITY; MOUSE SPERM; STRUCTURAL CHARACTERIZATION; UNFERTILIZED EGGS; OOCYTE MATURATION; ACROSOME REACTION; GLYCOPROTEIN ZP3; OVIDUCTAL ORIGIN; COMBINING SITE;
Keywords:
zona pellucida; glycoconjugates; lectins; Lewis X antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Ballesta, J Univ Murcia, Sch Med, Dept Cell Biol, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 Biol, E-30071 Murcia, Spain
Citazione:
M. Aviles et al., "Differential expression of glycoside residues in the mammalian zona pellucida", MOL REPROD, 57(3), 2000, pp. 296-308

Abstract

The mammalian zona pellucida is an extracellular matrix surrounding the oocyte, and is com posed of three major glycoproteins, ZP1, ZP2, and ZP3. Previous studies have suggested that the sperm receptor activity of the zona pellucida resides in specific oligosaccharide chains on the ZP3 glycoprotein. However, the nature of the terminal monosaccharide(s) on these glycosidicchains to which sperm bind is a matter of active debate. Evidence has beenpresented to support a role for at least three distinct monosaccharides insperm binding, alpha-galactose, L-fucose on Lewis X structures, and beta-N-acetylglucosamine. Previous studies have shown that beta-N-acetylglucosamine is uniformly distributed throughout the zona matrix. In this study, we have investigated the expression and distribution of alpha-galactose and fucose moieties during the maturation of the zona pellucida in mouse, rat, andhamster. Interestingly, alpha-galactose residues are expressed only duringlater stages of zona secretion and, consequently, are confined to the inner portions of the mature zona pellucida in mouse and rat. In hamster, alpha-galactose residues are only detectable in the zona pellucida of ovulated eggs, and are not found in ovarian oocytes. Fucosyl residues linked to LewisX glycosides are not detectable at any stage of zona maturation in these three species, whereas fucose linked to N-linked core oligosaccharides are present throughout the zona. These studies indicate a previously unappreciated heterogeneity in the composition of zona glycosides. The specific localization of alpha-galactose residues to the inner portions of the zona matrixsuggest a role in the later stages of sperm penetration through the zona. Finally, due to their absence from the zona surface, alpha-galactose and Lewis X fucosyl residues are not likely to be mediators of primary sperm binding. Mel. Reprod. Dev. 57:296-308, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 03:47:14