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Titolo:
Constitutive macropinocytosis in oncogene-transformed fibroblasts depends on sequential permanent activation of phosphoinositide 3-kinase and phospholipase C
Autore:
Amyere, M; Payrastre, B; Krause, U; Van Der Smissen, P; Veithen, A; Courtoy, PJ;
Indirizzi:
Univ Catholique Louvain, Cell Unit, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Univ Catholique Louvain, Hormone Unit, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Christian Duve Inst Cellular Pathol, B-1200 Brussels, Belgium Christian Duve Inst Cellular Pathol Brussels Belgium B-1200 els, Belgium Inst Natl Sante & Rech Med, F-31059 Toulouse, France Inst Natl Sante & Rech Med Toulouse France F-31059 1059 Toulouse, France
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 10, volume: 11, anno: 2000,
pagine: 3453 - 3467
SICI:
1059-1524(200010)11:10<3453:CMIOFD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED ENDOCYTOSIS; V-SRC; PHOSPHATIDYLINOSITOL 3-KINASE; DENDRITIC CELLS; DICTYOSTELIUM-DISCOIDEUM; ACTIN CYTOSKELETON; SIGNALING PATHWAY; RAT FIBROBLASTS; MAST-CELLS; MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Courtoy, PJ Univ Catholique Louvain, Cell Unit, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 els, Belgium
Citazione:
M. Amyere et al., "Constitutive macropinocytosis in oncogene-transformed fibroblasts depends on sequential permanent activation of phosphoinositide 3-kinase and phospholipase C", MOL BIOL CE, 11(10), 2000, pp. 3453-3467

Abstract

Macropinocytosis results from the closure of lamellipodia generated by membrane ruffling, thereby reflecting cortical actin dynamics. Both transformation of Rat-1 fibroblasts by v-Src or K-Ras and stable transfection for expression of dominant-positive, wild-type phosphoinositide 3-kinase (PI3K) regulatory subunit p85 alpha constitutively led to stress fiber disruption, cortical actin recruitment, extensive ruffling, and macropinosome formation,as measured by a selective acceleration of fluid-phase endocytosis. These alterations closely correlated with activation of PI3K and phosphatidylinositol-specific phospholipase C (PI-PLC), as assayed by S-phosphoinositide synthesis in situ and in vitro and inositol 1,4,5 trisphosphate steady-state levels, respectively; they were abolished by stable transfection of v-Src-transformed cells for dominant-negative truncated p85 alpha expression and by pharmacological inhibitors of PI3K and PI-PLC, indicating a requirement for both enzymes. Whereas PI3K activation resisted PI-PLC inhibition, PI-PLCactivation was abolished by a PI3K inhibitor and dominant-negative transfection, thus placing PI-PLC downstream of PI3K. Together, these data suggestthat permanent sequential activation of both PI3K and PI-PLC is necessary for the dramatic reorganization of the actin cytoskeleton in oncogene-transformed fibroblasts, resulting in constitutive ruffling and macropinocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 00:30:55