Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cbl-transforming variants trigger a cascade of molecular alterations that lead to epithelial mesenchymal conversion
Autore:
Fournier, TM; Lamorte, L; Maroun, CR; Lupher, M; Band, H; Langdon, W; Park, M;
Indirizzi:
McGill Univ, Royal Victoria Hosp, Dept Biochem, Montreal, PQ H3A 1A1, Canada McGill Univ Montreal PQ Canada H3A 1A1 chem, Montreal, PQ H3A 1A1, Canada McGill Univ, Royal Victoria Hosp, Dept Med, Montreal, PQ H3A 1A1, Canada McGill Univ Montreal PQ Canada H3A 1A1 Med, Montreal, PQ H3A 1A1, Canada McGill Univ, Royal Victoria Hosp, Dept Oncol, Montreal, PQ H3A 1A1, CanadaMcGill Univ Montreal PQ Canada H3A 1A1 ncol, Montreal, PQ H3A 1A1, Canada McGill Univ, Royal Victoria Hosp, Mol Oncol Grp, Montreal, PQ H3A 1A1, Canada McGill Univ Montreal PQ Canada H3A 1A1 Grp, Montreal, PQ H3A 1A1, Canada Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med, Boston, MA USA Harvard Univ Boston MA USA omens Hosp, Sch Med, Dept Med, Boston, MA USA Univ Western Australia, Queen Elizabeth II Med Ctr, Dept Pathol, Nedlands,WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 ands,WA 6907, Australia
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 10, volume: 11, anno: 2000,
pagine: 3397 - 3410
SICI:
1059-1524(200010)11:10<3397:CVTACO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; PHOSPHOTYROSINE-BINDING DOMAIN; PROTOONCOGENE C-CBL; INTEGRIN SIGNALING PATHWAY; TYROSINE KINASE; MET RECEPTOR; NEGATIVE REGULATOR; PHOSPHATIDYLINOSITOL 3-KINASE; DOCKING PROTEIN; ADAPTER PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Park, M McGill Univ, Royal Victoria Hosp, Dept Biochem, Montreal, PQ H3A 1A1, Canada McGill Univ Montreal PQ Canada H3A 1A1 ntreal, PQ H3A 1A1, Canada
Citazione:
T.M. Fournier et al., "Cbl-transforming variants trigger a cascade of molecular alterations that lead to epithelial mesenchymal conversion", MOL BIOL CE, 11(10), 2000, pp. 3397-3410

Abstract

Dispersal of epithelial cells is an important aspect of tumorigenesis, andinvasion. Factors such as hepatocyte growth factor induce the breakdown ofcell junctions and promote cell spreading and the dispersal of colonies ofepithelial cells, providing a model system to investigate the biochemical signals that regulate these events. Multiple signaling proteins are phosphorylated in epithelial cells during hepatocyte growth factor-induced cell dispersal, including c-Cbl, a protooncogene docking protein with ubiquitin ligase activity. We have examined the role of c-Cbl and a transforming variant (70z-Cb1) in epithelial cell dispersal. We show that the expression of 70z-Cb1 in Madin-Darby canine kidney epithelial cells resulted in the breakdown of cell-cell contacts and alterations in cell morphology characteristic of epithelial-mesenchymal transition. Structure-function studies revealed that the amino-terminal portion of c-Cbl, which corresponds to the Cbl phosphotyrosine-binding/Src homology domain 2, is sufficient to promote the morphological changes in cell shape. Moreover, a point mutation at Gly-306 abrogates the ability of the Cbl Src homology domain 2 to induce these morphological changes. Our results identify a role for Cbl in the regulation of epithelial-mesenchymal transition, including loss of adherens junctions, cell spreading, and the initiation of cell dispersal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:15:13