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Titolo:
The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomesand late endosomes
Autore:
Antonin, W; Holroyd, C; Tikkanen, R; Honing, S; Jahn, R;
Indirizzi:
Max Planck Inst Biophys Chem, Dept Neurobiol, D-3400 Gottingen, Germany Max Planck Inst Biophys Chem Gottingen Germany D-3400 Gottingen, Germany Univ Bonn, Inst Cell Biol, D-5300 Bonn, Germany Univ Bonn Bonn Germany D-5300 Bonn, Inst Cell Biol, D-5300 Bonn, Germany Univ Gottingen, Inst Biochem 2, D-3400 Gottingen, Germany Univ Gottingen Gottingen Germany D-3400 hem 2, D-3400 Gottingen, Germany
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 10, volume: 11, anno: 2000,
pagine: 3289 - 3298
SICI:
1059-1524(200010)11:10<3289:TREMHF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-FUSION; ENDOPLASMIC-RETICULUM; SYNAPTIC VESICLES; TETANUS-TOXIN; VESICULAR TRANSPORT; GOLGI TRANSPORT; IN-VITRO; PROTEIN; RAB5; COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Jahn, R Max Planck Inst Biophys Chem, Dept Neurobiol, POB 2841, D-3400 Gottingen, Germany Max Planck Inst Biophys Chem POB 2841 Gottingen Germany D-3400 ny
Citazione:
W. Antonin et al., "The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomesand late endosomes", MOL BIOL CE, 11(10), 2000, pp. 3289-3298

Abstract

Endobrevin/VAMP-8 is an R-SNARE localized to endosomes, but it is unknown in which intracellular fusion step it operates. Using subcellular fractionation and quantitative immunogold electron microscopy, we found that endobrevin/VAMP-8 is present on all membranes known to communicate with early endosomes, including the plasma membrane, clathrin-coated pits, late endosomes,and membranes of the trans-Golgi network. Affinity-purified antibodies that block the ability of endobrevin/VAMP-8 to form SNARE core complexes potently inhibit homotypic fusion of both early and late endosomes in vitro. Fabfragments were as active as intact immunoglobulin Gs. Recombinant endobrevin/VAMP-8 inhibited both fusion reactions with similar potency. We concludethat endobrevin/VAMP-8 operates as an R-SNARE in the homotypic fusion of early and late endosomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:20:20