Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: Cell cycle-regulated transcription factor Ace2p shows cell cycle-independent nucleocytoplasmic shuttling
Autore:
Jensen, TH; Neville, M; Rain, JC; McCarthy, T; Legrain, P; Rosbash, M;
Indirizzi:
Brandeis Univ, Dept Biol, Howard Hughes Med Inst, Waltham, MA 02454 USA Brandeis Univ Waltham MA USA 02454 Hughes Med Inst, Waltham, MA 02454 USA Inst Pasteur, CNRS, URA1300, Unite Genet Interact Macromol, F-75524 Paris 15, France Inst Pasteur Paris France 15 Interact Macromol, F-75524 Paris 15, France
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 21, volume: 20, anno: 2000,
pagine: 8047 - 8058
SICI:
0270-7306(200011)20:21<8047:IONSCP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPORT; INFECTIOUS-ANEMIA VIRUS; REV ACTIVATION DOMAIN; IMMUNODEFICIENCY-VIRUS; LEPTOMYCIN-B; NUCLEOSIDE TRIPHOSPHATASE; SUBCELLULAR-LOCALIZATION; OXIDATIVE STRESS; CAPPING ENZYME; PORE COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Rosbash, M Brandeis Univ, Dept Biol, Howard Hughes Med Inst, 415 South St,Waltham, MA 02454 USA Brandeis Univ 415 South St Waltham MA USA 02454 m, MA 02454 USA
Citazione:
T.H. Jensen et al., "Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: Cell cycle-regulated transcription factor Ace2p shows cell cycle-independent nucleocytoplasmic shuttling", MOL CELL B, 20(21), 2000, pp. 8047-8058

Abstract

Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the NES receptor CRM1/Crm1p. We have carried out a yeast two-hybrid screen with Crm1p as a bait. The Crm1p-interacting clones were subscreened for nuclear export activity in a visual assay utilizing the Crm1p-inhibitor leptomycin B (LMB). This approach identified three Saccharomyces cerevisiae proteins not previously known to have nuclear export activity. These proteins are the 5' RNA triphosphatase Ctl1p, the cell cycle-regulated transcription factor Ace2p, and a protein encoded by the previously uncharacterized open reading frame YDR499W. Mutagenesis analysis show that YDR499Wp contains an NES that conforms to the consensus sequence for leucine rich NESs. Mutagenesis of Ctl1p and Ace2p were unable to identify specific NES residues, However, a 29-amino-acid region of Ace2p, rich in hydrophobic residues, contains nuclear export activity. Ace2p accumulates in the nucleus at the end of mitosis and activates early-G(1)-specific genes. We now provide evidence that Ace2p is nuclear not only in late M-early G(1) but also during other stages of the cell cycle. This feature of Ace2p localization explains its ability to activate genes such as CUP1, which are not expressed in a cell cycle-dependent manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 03:14:05