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Titolo:
Identification of upregulated genes in scrapie-infected brain tissue
Autore:
Riemer, C; Queck, I; Simon, D; Kurth, R; Baier, M;
Indirizzi:
Robert Koch Inst, D-13353 Berlin, Germany Robert Koch Inst Berlin Germany D-13353 ch Inst, D-13353 Berlin, Germany
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 21, volume: 74, anno: 2000,
pagine: 10245 - 10248
SICI:
0022-538X(200011)74:21<10245:IOUGIS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CREUTZFELDT-JAKOB-DISEASE; PRION PROTEIN-FRAGMENT; CENTRAL-NERVOUS-SYSTEM; ALZHEIMERS-DISEASE; SUBTRACTIVE HYBRIDIZATION; ORAL INFECTION; MURINE SCRAPIE; MOUSE-BRAIN; EXPRESSION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Baier, M Robert Koch Inst, Nordufer 20, D-13353 Berlin, Germany Robert Koch Inst Nordufer 20 Berlin Germany D-13353 lin, Germany
Citazione:
C. Riemer et al., "Identification of upregulated genes in scrapie-infected brain tissue", J VIROLOGY, 74(21), 2000, pp. 10245-10248

Abstract

The pathogenesis of scrapie, and of neurodegenerative diseases in general,is still insufficiently understood and is therefore being intensely researched. There is abundant evidence that the activation of glial cells precedes neurodegeneration and may thus play an important role in disease development and progression. The identification of genes with altered expression patterns in the diseased brain may provide insight on the molecular level into the process which ultimately leads to neuronal loss. Differentially expressed genes in scrapie-infected brain tissue were enriched by the suppression subtractive hybridization technique, molecularly cloned, and further characterized. Northern blotting and nucleotide sequencing confirmed the identities of 19 upregulated genes, 11 of which were unknown to be affected by scrapie. A considerable number of these 19 genes, namely those encoding interferon-inducible protein 10 (IP-10), 2',5'-oligo(A) synthetase, Mx protein, IIGP protein, major histocompatibility complex classes I and II, complement, and beta(2)-microglobulin, were inducible by interferons (IFNs), suggesting that an IFN response is a possible mechanism of gene activation in scrapie. Among the newly found genes, that coding for 2',5'-oligo(A) synthetase is of special interest because it could contribute to the apoptotic loss ofneuronal cells via RNase L activation. In addition, upregulation of the chemokine IP-10 and B-lymphocyte chemoattractant mRNAs was seen at relativelyearly stages of the disease and was sustained throughout disease development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:19:01