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Titolo:
A comparison of cytology and fluorescence in situ hybridization for the detection of urothelial carcinoma
Autore:
Halling, KC; King, W; Sokolova, IA; Meyer, RG; Burkhardt, HM; Halling, AC; Cheville, JC; Sebo, TJ; Ramakumar, S; Stewart, CS; Pankratz, S; OKane, DJ; Seelig, SA; Lieber, MM; Jenkins, RB;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 thol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Urol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Urol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Biostat, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 stat, Rochester, MN 55905 USA Vysis Inc, Downers Grove, IL USA Vysis Inc Downers Grove IL USAVysis Inc, Downers Grove, IL USA
Titolo Testata:
JOURNAL OF UROLOGY
fascicolo: 5, volume: 164, anno: 2000,
pagine: 1768 - 1775
SICI:
0022-5347(200011)164:5<1768:ACOCAF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSITIONAL-CELL CARCINOMA; SUPERFICIAL BLADDER-CANCER; VOIDED URINE CYTOLOGY; BTA TRAK ASSAY; IN-SITU; FLOW-CYTOMETRY; TELOMERASE ACTIVITY; MULTICENTER TRIAL; INTERPHASE CYTOGENETICS; CLINICAL-EVALUATION;
Keywords:
carcinoma; urothelium; in situ hybridization; fluorescence; bladder; cytology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Halling, KC Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, 200 1st St SW,Hilton 800, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW,Hilton 800 Rochester MN USA 55905
Citazione:
K.C. Halling et al., "A comparison of cytology and fluorescence in situ hybridization for the detection of urothelial carcinoma", J UROL, 164(5), 2000, pp. 1768-1775

Abstract

Purpose: We determine the relative sensitivities of cytology and fluorescence in situ hybridization (FISH) for the detection of urothelial carcinoma. Materials and Methods: A mixture of fluorescent labeled probes to the centromeres of chromosomes 3, 7 and 17, and band 9p21 (P16/CDKN2A gene) was used to assess urinary cells for chromosomal abnormalities indicative of malignancy. A total of 280 urine specimens from 265 patients, including 150 witha history of urothelial carcinoma and 115 without a history of urothelial carcinoma, were analyzed. FISH analysis was performed without prior knowledge of clinical findings, that is biopsy, cystoscopy and cytology results. Apositive result was defined as 5 or more urinary cells with gains of 2 or more chromosomes. Results: A total of 75 biopsies showed urothelial carcinoma at FISH analysis among the 265 patients. The sensitivity of urine cytology for pTa (36 cases), pTis (18) and pT1-pT4 (15) tumors was 47%, 78% and 60%, respectively,for an overall sensitivity of 58%. The sensitivity of FISH for pTa (37 cases), pTis (17) and pT1-pT4 (19) tumors was 65%, 100% and 95%, respectively,for an overall sensitivity of 81%. FISH was significantly more sensitive than cytology for pTis (p = 0.046), pT1-pT4 (p = 0.025), grade 3 (p = 0.003)and all tumors (p = 0.001). The specificity of cytology and FISH among patients without cystoscopic evidence of urothelial carcinoma and no history of urothelial carcinoma was 98% and 96%, respectively (p = 0.564). Conclusions: The sensitivity of FISH for the detection of urothelial carcinoma is superior to that of cytology, and the specificity of FISH and cytology for urothelial carcinoma are not significantly different. Further prospective studies are required but FISH has the potential to improve significantly the management of urothelial carcinoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:01:43