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Titolo:
Molecular characterization of a water-soluble chlorophyll protein from main veins of Japanese radish
Autore:
Shinashi, K; Satoh, H; Uchida, A; Nakayama, K; Okada, M; Oonishi, I;
Indirizzi:
Toho Univ, Fac Sci, Dept Biomol Sci, Funabashi, Chiba 2748510, Japan Toho Univ Funabashi Chiba Japan 2748510 , Funabashi, Chiba 2748510, Japan
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 3, volume: 157, anno: 2000,
pagine: 255 - 262
SICI:
0176-1617(200009)157:3<255:MCOAWC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRASSICA-NAPUS LEAVES; A/B-PROTEIN; DROUGHT; CAULIFLOWER; EXPRESSION; STRESS; BND22;
Keywords:
AtDr4; BnD22; Brassicaceae; Kunitz proteinase inhibitor; Raphanus sativus L. var.; hortensis WSCP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Satoh, H Toho Univ, Fac Sci, Dept Biomol Sci, 2-2-1 Miyama, Funabashi, Chiba 2748510, Japan Toho Univ 2-2-1 Miyama Funabashi Chiba Japan 2748510 48510, Japan
Citazione:
K. Shinashi et al., "Molecular characterization of a water-soluble chlorophyll protein from main veins of Japanese radish", J PLANT PHY, 157(3), 2000, pp. 255-262

Abstract

A water-soluble chlorophyll (Chl) protein (WSCP) from the main veins of the Japanese radish, Raphanus sativus L. var. hortensis, was purified and characterized. We sequenced 26 N-terminal residues of the Raphanus-WSCP and found a complete signature motif of Kunitz proteinase inhibitors, as in WSCPsfrom other Brassicaceae plants. The Raphanus-WSCP sequence shared 92 % identity with that of a Lepidium-WSCP; however, the antibody against recombinant Lepidium-WSCP did not recognize the Raphanus-WSCP, indicating that the N-terminal region may not be a major antigenic epitope. On the other hand, while the absorption spectrum of the red band of the Raphanus-WSCP was distinct from that of the Lepidium-WSCP, it was similar to that of Brassica-WSCP. Spectrophotometric and spectrofluorometric analyses of Raphanus-WSCP from the main veins revealed that the red band was comprised of four Chi a and one Chi b forms. In addition, this WSCP contained an intense absorption component with a peak at 698 nm, which is not prominent in WSCP from Raphanus leaflets,As an initial step in determining its molecular structure, the Raphanus-WSCP was crystallized. A green. parallelepiped, single crystal was obtained. This is the first characterization and crystallization of WSCP from the genus Raphanus, which is the third documented genus possessing WSCP in Brassicaceae plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 10:24:01