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Titolo:
Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells
Autore:
Kukutsch, NA; Rossner, S; Austyn, JM; Schuler, G; Lutz, MB;
Indirizzi:
Univ Erlangen Nurnberg, Dept Dermatol, D-91052 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91052 -91052 Erlangen, Germany Univ Oxford, John Radcliffe Hosp, Nuffield Dept Surg, Oxford OX3 9DU, England Univ Oxford Oxford England OX3 9DU ld Dept Surg, Oxford OX3 9DU, England
Titolo Testata:
JOURNAL OF INVESTIGATIVE DERMATOLOGY
fascicolo: 3, volume: 115, anno: 2000,
pagine: 449 - 453
SICI:
0022-202X(200009)115:3<449:FAKOMC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIVING CELLS; HALF-LIFE; MOLECULES; ANTIGEN;
Keywords:
antigen presentation; dendritic cell; MHC class I-peptide complexes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Lutz, MB Univ Erlangen Nurnberg, Dept Dermatol, Hartmannstr 14, D-91052 Erlangen, Germany Univ Erlangen Nurnberg Hartmannstr 14 Erlangen Germany D-91052 y
Citazione:
N.A. Kukutsch et al., "Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells", J INVES DER, 115(3), 2000, pp. 449-453

Abstract

Dendritic cells (DC) are professional antigen-presenting cells that are able to induce primary T cell responses. Therefore, several strategies employpeptide-pulsed DC in tumor immunotherapy. For efficient antigen presentation and induction of an immune response by DC the number and stability of MHC I-peptide complexes is crucial. We studied this issue by using the antibody 25-D1.16 that specifically detects OVA peptide SIINFEKL in conjunction with H-2 K-b molecules, and determined its kinetics on mature and immature bone marrow-derived murine DC. Optimal peptide loading was reached after 8-16 h at 50 mu M peptide pulse, and was comparable in serum-free versus serum-containing medium. Stimulation of DC with LPS or Poly I:C, and to a lesserextent TNF-alpha, upregulated the total number of surface MHC I molecules and thus improved peptide loading. Pulse-chase experiments revealed a constant half-life of peptide/K-b complexes independent of preceding DC stimulation or their maturation stage. The duration of peptide/K-b complex expression on mature DC, however, could be extended from 24 h to 72 h when the cultures were pretreated with LPS or Poly I:C, but not TNF-alpha. These data might have important implications for the clinical application of peptide-pulsed DC in tumor immunotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 11:25:32