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Titolo:
Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells
Autore:
Csonka, E; Cserpan, I; Fodor, K; Hollo, G; Katona, R; Kereso, J; Praznovszky, T; Szakal, B; Telenius, A; deJong, G; Udvardy, A; Hadlaczky, G;
Indirizzi:
Hungarian Acad Sci, Biol Res Ctr, Genet Inst, H-6701 Szeged, Hungary Hungarian Acad Sci Szeged Hungary H-6701 et Inst, H-6701 Szeged, Hungary Chromos Mol Syst Inc, Burnaby, BC, Canada Chromos Mol Syst Inc Burnaby BCCanada Mol Syst Inc, Burnaby, BC, Canada Hungarian Acad Sci, Biol Res Ctr, Inst Biochem, H-6701 Szeged, Hungary Hungarian Acad Sci Szeged Hungary H-6701 Biochem, H-6701 Szeged, Hungary
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 18, volume: 113, anno: 2000,
pagine: 3207 - 3216
SICI:
0021-9533(200009)113:18<3207:NGOHSD>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ALPHA-SATELLITE; ACROCENTRIC CHROMOSOMES; GENE-CLUSTER; RNA GENES; SEQUENCE; REPEAT; FRAGMENTATION; CENTROMERE; MINICHROMOSOME; AMPLIFICATION;
Keywords:
human satellite DNA-based artificial chromosome; rDNA; large-scale amplification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Hadlaczky, G Hungarian Acad Sci, Biol Res Ctr, Genet Inst, POB 521, H-6701Szeged, Hungary Hungarian Acad Sci POB 521 Szeged Hungary H-6701 ed, Hungary
Citazione:
E. Csonka et al., "Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells", J CELL SCI, 113(18), 2000, pp. 3207-3216

Abstract

An in vivo approach has been developed for generation of artificial chromosomes, based on the induction of intrinsic, large-scale amplification mechanisms of mammalian cells. Here, we describe the successful generation of prototype human satellite DNA-based artificial chromosomes via amplification-dependent de novo chromosome formations induced by integration of exogenousDNA sequences into the centromeric/rDNA regions of human acrocentric chromosomes. Subclones with mitotically stable de novo chromosomes were established, which allowed the initial characterization and purification of these artificial chromosomes. Because of the low complexity of their DNA content, they may serve as a useful tool to study the structure and function of higher eukaryotic chromosomes. Human satellite DNA-based artificial chromosomescontaining amplified satellite DNA, rDNA, and exogenous DNA sequences wereheterochromatic, however, they provided a suitable chromosomal environmentfor the expression of the integrated exogenous genetic material, We demonstrate that induced de novo chromosome formation is a reproducible and effective methodology in generating artificial chromosomes from predictable sequences of different mammalian species, Satellite DNA-based artificial chromosomes formed by induced large-scale amplifications on the short arm of human acrocentric chromosomes may become safe or low risk vectors in gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 10:19:45