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Titolo:
Versatile transcription of biphenyl catabolic bph operon in Pseudomonas pseudoalcaligenes KF707
Autore:
Watanabe, T; Inoue, R; Kimura, N; Furukawa, K;
Indirizzi:
Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Appl Microbiol Lab, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 Microbiol Lab, Fukuoka 8128581, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 40, volume: 275, anno: 2000,
pagine: 31016 - 31023
SICI:
0021-9258(20001006)275:40<31016:VTOBCB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP STRAIN KKS102; GRAM-NEGATIVE BACTERIA; RHODOCOCCUS SP M5; NUCLEOTIDE-SEQUENCE; ENCODING BIPHENYL; CHLOROBIPHENYL DEGRADATION; TRANSPOSON MUTAGENESIS; FUNCTIONAL-ANALYSIS; SOIL BACTERIA; GENE-CLUSTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Appl Microbiol Lab, Hakozaki 6-10-1, Fukuoka 8128581, Japan Kyushu Univ Hakozaki 6-10-1 Fukuoka Japan 8128581 8581, Japan
Citazione:
T. Watanabe et al., "Versatile transcription of biphenyl catabolic bph operon in Pseudomonas pseudoalcaligenes KF707", J BIOL CHEM, 275(40), 2000, pp. 31016-31023

Abstract

Pseudomonas pseudoalcaligenes KF707 possesses a chromosomally encoded bph gene cluster responsible for the catabolism of biphenyl/polychlorinated biphenyls. The gene cluster consists of (orf0)bphA1A-2(orf3)bphA3A4BCX0X1X2X3D. We studied the role of orf0 and transcription in the KF707 bph operon, Primer extension analyses revealed that at least as many as six transcriptional initiation sites exist upstream of orf0, bphA1, bphX0, bphX1, and bphD, including two upstream of bphD. The orf0-disruptant failed to grow on biphenyl but accumulated large amounts of the biphenyl ring meta-cleavage yellowcompound (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate). Western blot analysis revealed that ORF0 protein is inducibly expressed in KF707 in the presence of biphenyl. Gel shift assay revealed that ORFO directly binds to the orf0 operator region, This binding was greatly enhanced by addition of the biphenyl ring meta-cleavage yellow compound. These results indicated that orf0, bphA1A2(orf3)bphA3A4BC and bphX0X1X2X3D are independently transcribed, and that ORFO protein belonging to the GntR family is involved in the regulation of the bph operon in KF707 and is absolutely required for the expression of orf0 and bphX0X1X2X3D.

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Documento generato il 20/01/21 alle ore 03:16:26