Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids
Autore:
Ranson, H; Jensen, B; Vulule, JM; Wang, X; Hemingway, J; Collins, FH;
Indirizzi:
Cardiff Univ, Main Coll, Sch Biosci, Cardiff CF1 3TL, S Glam, Wales Cardiff Univ Cardiff S Glam Wales CF1 3TL Cardiff CF1 3TL, S Glam, Wales Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA Univ Notre Dame Notre Dame IN USA 46556 iol Sci, Notre Dame, IN 46556 USA Kenya Med Res Inst, Vector Biol & Control Res Ctr, Kisumu, Kenya Kenya MedRes Inst Kisumu Kenya r Biol & Control Res Ctr, Kisumu, Kenya
Titolo Testata:
INSECT MOLECULAR BIOLOGY
fascicolo: 5, volume: 9, anno: 2000,
pagine: 491 - 497
SICI:
0962-1075(200010)9:5<491:IOAPMI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
KNOCKDOWN-RESISTANCE; IMPREGNATED BEDNETS; KDR; MOSQUITOS; COMPLEX; INSECTICIDES;
Keywords:
pyrethroid resistance; DDT resistance; Anopheles gambiae; sodium channel; kdr;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Ranson, H Cardiff Univ, Main Coll, Sch Biosci, Cardiff CF1 3TL, S Glam, Wales Cardiff Univ Cardiff S Glam Wales CF1 3TL F1 3TL, S Glam, Wales
Citazione:
H. Ranson et al., "Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids", INSEC MOL B, 9(5), 2000, pp. 491-497

Abstract

A field trial of permethrin-impregnated bednets and curtains was initiatedin Western Kenya in 1990, and a strain of Anopheles gambiae showing reduced susceptibility to permethrin was colonized from this site in 1992. A leucine-phenylalanine substitution at position 1014 of the voltage-gated sodiumchannel is associated with resistance to permethrin and DDT in many insectspecies, including Anopheles gambiae from West Africa. We cloned and sequenced a partial sodium channel cDNA from the Kenyan permethrin-resistant strain and we identified an alternative substitution (leucine to serine) at the same position, which is linked to the inheritance of permethrin resistance in the F-2 progeny of genetic crosses between susceptible and resistant individuals. The diagnostic polymerase chain reaction (PCR) developed by Martinez-Torres et al. [(1998) Insect Mol Biol 7: 179-184] to detect kdr alleles in field populations of An. gambiae will not detect the Kenyan allele and hence reliance on this assay may lead to an underestimate of the prevalence of pyrethroid resistance in this species. We adapted the diagnostic PCR to detect the leucine-serine mutation and with this diagnostic we were ableto demonstrate that this kdr allele was present in individuals collected from the Kenyan trial site in 1986, prior to the introduction of pyrethroid-impregnated bednets. The An. gambiae sodium channel was physically mapped to chromosome 2L, division 20C. This position corresponds to the location ofa major quantitative trait locus determining resistance to permethrin in the Kenyan strain of An. gambiae.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:31:45