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Titolo:
Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells
Autore:
Negre, D; Mangeot, PE; Duisit, G; Blanchard, S; Vidalain, PO; Leissner, P; Winter, AJ; Rabourdin-Combe, C; Mehtali, M; Moullier, P; Darlix, JL; Cosset, FL;
Indirizzi:
Ecole Normale Super Lyon, INSERM U412, LVRTG, F-69364 Lyon 07, France Ecole Normale Super Lyon Lyon France 07 , LVRTG, F-69364 Lyon 07, France IFR 74, INSERM U412, LaboRetro, Unite Virol Humaine, F-69364 Lyon, France IFR 74 Lyon France F-69364 ro, Unite Virol Humaine, F-69364 Lyon, France U503 INSERM IFR 74, Immunobiol Fondamentale & Clin, F-69364 Lyon, France U503 INSERM IFR 74 Lyon France F-69364 tale & Clin, F-69364 Lyon, France CHU Nantes, Hotel Dieu, Lab Therapie Genique, F-44035 Nantes 01, France CHU Nantes Nantes France 01 Therapie Genique, F-44035 Nantes 01, France Transgene SA, Strasbourg, France Transgene SA Strasbourg FranceTransgene SA, Strasbourg, France
Titolo Testata:
GENE THERAPY
fascicolo: 19, volume: 7, anno: 2000,
pagine: 1613 - 1623
SICI:
0969-7128(200010)7:19<1613:CONSLV>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE LEUKEMIA-VIRUS; MEDIATED GENE-TRANSFER; IN-VIVO; NONDIVIDING CELLS; RETROVIRAL VECTORS; NUCLEAR-LOCALIZATION; HIGHLY EFFICIENT; TYPE-1 PARTICLES; MATRIX PROTEIN; PRODUCER CELL;
Keywords:
lentiviral vectors; retroviruses; dendritic cells; pseudotypes; safety; packaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Cosset, FL Ecole Normale Super Lyon, INSERM U412, LVRTG, 6 Allee Italie, F-69364 Lyon07, France Ecole Normale Super Lyon 6 Allee Italie Lyon France 07 France
Citazione:
D. Negre et al., "Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells", GENE THER, 7(19), 2000, pp. 1613-1623

Abstract

We describe the generation and the characterization of new lentiviral Vectors derived from SIVmac251, a simian immunodeficiency virus (SIV). A methodical approach was used to engineer both efficient and safe packaging constructs allowing the production of SIV viral core proteins. SIV-vectors encoding GFP (green fluorescent protein) were generated as VSV-G-pseudotyped particles upon transient expression of the vector construct and helper functions in 293 cells. The SIV vectors were able to transduce efficiently various target cell types at low multiplicity of infection, including monocyte-differentiated human dendritic cells (DCs) which retained their capacity to differentiate into mature DCs after gene transfer. Transduction of the DCs by the SIV vectors was prevented when infections were performed in the presence of AZT, a reverse-transcriptase inhibitor. After gene transfer, expression of the GFP in the target cells remained constant after several weeks, indicating that the vectors had been stably integrated into the genome of the host cells. Preparations of SIV vectors were systematically checked for theabsence of replication-competent and recombinant retroviruses but remainednegative, suggesting the innocuousness of these novel gene delivery vectors. Side-to-side comparisons with Vectors derived from HIV-1 (human immunodeficiency virus) indicated that the SIV vectors were equally potent in transducing proliferating target cells. Finally, we have determined the infectivity of SIV vectors pseudotyped with surface glycoproteins of several membrane-enveloped viruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 07:00:38