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Titolo:
Kinesin is involved in regulation of rat pancreatic amylase secretion
Autore:
Ueda, N; Ohnishi, H; Kanamaru, C; Suzuki, J; Tsuchida, T; Mashima, H; Yasuda, H; Fujita, T;
Indirizzi:
Univ Tokyo, Sch Med, Dept Internal Med 4, Bunkyo Ku, Tokyo 1128688, Japan Univ Tokyo Tokyo Japan 1128688 al Med 4, Bunkyo Ku, Tokyo 1128688, Japan
Titolo Testata:
GASTROENTEROLOGY
fascicolo: 4, volume: 119, anno: 2000,
pagine: 1123 - 1131
SICI:
0016-5085(200010)119:4<1123:KIIIRO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLS; TRANSPORT; PHOSPHORYLATION; EXOCYTOSIS; PROTEINS; BINDING; RELEASE; KIDNEY; CHAINS; MOTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Ohnishi, H Univ Tokyo, Sch Med, Dept Internal Med 4, Bunkyo Ku, 3-28-6 Mejirodai, Tokyo 1128688, Japan Univ Tokyo 3-28-6 Mejirodai Tokyo Japan 1128688 1128688, Japan
Citazione:
N. Ueda et al., "Kinesin is involved in regulation of rat pancreatic amylase secretion", GASTROENTY, 119(4), 2000, pp. 1123-1131

Abstract

Background & Aims: Kinesin has recently been localized to zymogen granulesof pancreatic acini and is suggested to participate in exocytosis of exocrine pancreas, We examined the function of kinesin in regulated exocytosis of pancreatic acini in this study, Methods: Kinesin function in exocytosis was examined by introducing hexahistidine-tagged recombinant kinesin proteinand antikinesin monoclonal antibody into streptolysin-O-permeabilized acini. Intracellular localization of introduced recombinant kinesin was investigated by immunohistochemistry. Interaction between recombinant kinesin and the microtubule network was confirmed by nocodazole pretreatment of acini, Kinesin regulation by secretagogues was investigated by examining their effect on adenosine triphosphatase (ATPase) activity of endogenous kinesin, Results: Recombinant kinesin enhanced calcium-stimulated amylase release fromstreptolysin-O-permeabilized acini, Introduced recombinant kinesin was localized to both the microtubule network and zymogen granule, Nocodazole pretreatment of acini abolished the enhancing effect of recombinant kinesin on calcium-stimulated amylase release. Antikinesin antibody inhibited amylase release stimulated by the combination of calcium and cyclic adenosine monophosphate (cAMP) but not that stimulated by calcium alone, Secretin and 8-bromo-cAMP increased ATPase activity of endogenous kinesin. Conclusions: Kinesin plays a stimulatory role in regulated exocytosis of pancreatic acini and is involved in stimulus-secretion coupling through a cAMP-dependent pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:12:27