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Titolo:
Orphanin FQ causes contractions via inhibiting purinergic pathway in the rat colon
Autore:
Takahashi, T; Bagnol, D; Schneider, D; Mizuta, Y; Ishiguchi, T; LePard, K; Galligan, JJ; Watson, SJ; Owyang, C;
Indirizzi:
Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 nternal Med, Ann Arbor, MI 48109 USA Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 th Res Inst, Ann Arbor, MI 48109 USA Michigan State Univ, Dept Pharmacol & Toxicol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 xicol, E Lansing, MI 48824 USA
Titolo Testata:
GASTROENTEROLOGY
fascicolo: 4, volume: 119, anno: 2000,
pagine: 1054 - 1063
SICI:
0016-5085(200010)119:4<1054:OFCCVI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE-INTESTINAL-PEPTIDE; MOUSE VAS-DEFERENS; NITRIC-OXIDE; SPINAL-CORD; NOCICEPTIN RECEPTOR; ENDOGENOUS LIGAND; ORL(1) RECEPTOR; ORL1 RECEPTOR; DISTAL COLON; IN-VITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Takahashi, T Univ Michigan Hlth Syst, Taubman Ctr 3912, Ann Arbor, MI 48109 USA Univ Michigan Hlth Syst Ann Arbor MI USA 48109 MI 48109 USA
Citazione:
T. Takahashi et al., "Orphanin FQ causes contractions via inhibiting purinergic pathway in the rat colon", GASTROENTY, 119(4), 2000, pp. 1054-1063

Abstract

Background & Aims: We have previously shown that orphanin FQ (OFQ) preferentially stimulates muscle contraction in the rat colon. However, the mechanism of action of OFQ remains unclear. Methods: We studied the effects of OFQ on muscle contractions and inhibitory junction potentials (IJPs) in rat colon. The site of action of OFQ was also investigated by in situ hybridization of OFQ receptors. Results: OFQ (10(-10) to 10(-6) mol/L) caused circular muscle contractions that were blocked by tetrodotoxin (10(-7) mol/L), suggesting the contractions were nerve mediated. Suramin (a nonselective P-2-purinoceptor antagonist; 10(-4) mol/L) and reactive blue 2 (a P-2Y-purinoceptor antagonist; 3 x 10(-5) mol/L), but not pyridoxalphosphate-6-azophenyl-2',4' disulfonic acid (PPADS; a P-2X-purinoceptor antagonist; 3 x 10(-5) mol/L), abolished OFQ-induced colonic contractions. Focal stimulation of interganglionic fiber tracts evoked biphasic IJPs in colonic circular muscle cells. Suramin and reactive blue 2 inhibited the peak amplitude of the IJP, whereas PPADS had no effect. Cumulative addition of OFQ (10(-10) to 10(-6) mol/L) significantly inhibited the IJPs. In situ hybridization revealed that OFQ receptor messenger RNA was expressed in the colonic myenteric plexus but not in the smooth muscle cells, suggesting that the site of action of OFQis neuronal. Conclusions: These results suggest that OFQ causes muscle contractions by inhibiting purinergic inhibitory motorneurons in the rat colon.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:07:26