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Titolo:
Polyphasic microbial community analysis of petroleum hydrocarbon-contaminated soils from two northern Canadian communities
Autore:
Juck, D; Charles, T; Whyte, LG; Greer, CW;
Indirizzi:
McGill Univ, St Anne De Bellevue, PQ H9X 3V9, Canada McGill Univ St Anne De Bellevue PQ Canada H9X 3V9 vue, PQ H9X 3V9, Canada Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 ol, Waterloo, ON N2L 3G1, Canada Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, CanadaNatl Res Council Canada Montreal PQ Canada H4P 2R2 al, PQ H4P 2R2, Canada
Titolo Testata:
FEMS MICROBIOLOGY ECOLOGY
fascicolo: 3, volume: 33, anno: 2000,
pagine: 241 - 249
SICI:
0168-6496(200009)33:3<241:PMCAOP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
16S RIBOSOMAL-RNA; GRADIENT GEL-ELECTROPHORESIS; LEVEL PHYSIOLOGICAL PROFILES; BACTERIAL COMMUNITIES; LOW-TEMPERATURES; CRUDE-OIL; PCR; BIODEGRADATION; POPULATIONS; DIVERSITY;
Keywords:
cold-adapted bacterium; microbial community analysis; denaturing gradient gel electrophoresis; Biolog; petroleum hydrocarbon contamination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Greer, CW McGill Univ, Macdonald Campus,21 111 Lakeshore Blvd, St Anne De Bellevue, PQ H9X 3V9, Canada McGill Univ Macdonald Campus,21 111 Lakeshore Blvd St Anne De Bellevue PQ Canada H9X 3V9
Citazione:
D. Juck et al., "Polyphasic microbial community analysis of petroleum hydrocarbon-contaminated soils from two northern Canadian communities", FEMS MIC EC, 33(3), 2000, pp. 241-249

Abstract

The cold-adapted bacterial communities in petroleum hydrocarbon-contaminated and non-impacted soils from two northern Canadian environments, Kuujjuaq, Que,, and Alert, Nunavut, were analyzed using a polyphasic approach. Denaturing gradient gel electrophoresis (DGGE) separation of 16S rDNA PCR fragments from soil total community DNA revealed a high level of bacterial diversity, as estimated by the total number of bands visualized. Dendrogram analysis clustered the sample sites on the basis of geographical location. Comparison of the overall microbial molecular diversity suggested that in the Kuujjuaq sites, contamination negatively impacted diversity whereas in the Alert samples, diversity was maintained or increased as compared to uncontaminated controls. Extraction and sequencing analysis of selected 16S rDNA bands demonstrated a range of similarity of 86-100% to reference organisms, with 63.6% of the bands representing high G+C Gram-positive organisms in theorder Actinomycetales and 36.4% in the class Proteobacteria. Community level physiological profiles generated using Bioiog GN plates were analyzed bycluster analysis. Based on substrate oxidation rates, the samples clustered into groups similar to those of the DGGE dendrograms, i.e. separation based upon geographic origin. The coinciding results reached using culture-independent and -dependent analyses reinforces the conclusion that geographical origin of the samples, rather than petroleum contamination level, was more important in determining species diversity within these cold-adapted bacterial communities. Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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Documento generato il 19/09/20 alle ore 09:03:38