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Titolo:
Interactions between mitochondrial proteins and lipid peroxidation products in the maintenance of the glomerular filtration barrier in the in vitro perfused kidney
Autore:
Wang, SX; Solin, ML; Ahola, H; Luimula, P; Holthofer, H;
Indirizzi:
Univ Helsinki, Haartman Inst, Dept Bacteriol & Immunol, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 ol, FIN-00014 Helsinki, Finland
Titolo Testata:
EXPERIMENTAL NEPHROLOGY
fascicolo: 1, volume: 9, anno: 2001,
pagine: 14 - 20
SICI:
1018-7782(2001)9:1<14:IBMPAL>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONGENITAL NEPHROTIC SYNDROME; CYTOCHROME-C-OXIDASE; COMPLEX-I DEFICIENCY; HEYMANN NEPHRITIS; FINNISH TYPE; RAT-KIDNEY; EXPRESSION; MECHANISMS; DISEASE; INJURY;
Keywords:
isolated perfused kidney; rotenone; antimycin A; mitochondria; cytochrome-c oxidase; lipid peroxidation products; proteinuria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Holthofer, H Univ Helsinki, Haartman Inst, Dept Bacteriol & Immunol, Box 21,Haartmaninkatu 3, FIN-00014 Helsinki, Finland Univ Helsinki Box 21,Haartmaninkatu 3 Helsinki Finland FIN-00014
Citazione:
S.X. Wang et al., "Interactions between mitochondrial proteins and lipid peroxidation products in the maintenance of the glomerular filtration barrier in the in vitro perfused kidney", EXP NEPHROL, 9(1), 2001, pp. 14-20

Abstract

Background: The fourth complex of the mitochondrial respiratory chain, cytochrome-c oxidase (CytC) consists of thirteen both mitochondrially and nuclearly encoded subunits, which are differently regulated in proteinuric kidneys. The effect of mitochondrial involvement on proteinuria is not known. Methods: We set up an in vitro kidney perfusion model to study the direct effect of inhibitors of the mitochondrial respiratory chain, rotenone and antimycin A, on the glomerular filtration barrier by using immunohistochemistry and Northern blotting and quantitating the resulting proteinuria. Results: Rapid onset of proteinuria and characteristic changes in CytC subunits were seen in the perfused kidneys. Urinary protein excretion increased significantly in the rotenone- and antimycin-A-treated groups during perfusion. Downregulation of CytC subunits I and IV was similarly found in the groups treated with rotenone and antimycin A, while increases in the lipid peroxidation (LPO) products malondialdehyde and 4-hydroxynonenal which reflect mitochondrial damage, were observed. Conclusions: These data show rapid changesin mitochondrial proteins and induction of proteinuria in response to exposure to mitochondrial inhibitors. Together with the concomitant increase inlocal LPO products, these results suggest that continuous maintenance of aproper energy balance is important to maintain the glomerular filtration barrier. Copyrieht (C) 2000 S. Karger AG, Basel.

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Documento generato il 30/03/20 alle ore 18:08:04