Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A high-performance liquid chromatography assay for yohimbine HCl analysis
Autore:
Mittal, S; Alexander, KS; Dollimore, D;
Indirizzi:
Univ Toledo, Coll Pharm, Toledo, OH 43606 USA Univ Toledo Toledo OH USA 43606 Toledo, Coll Pharm, Toledo, OH 43606 USA Univ Toledo, Dept Chem, Toledo, OH 43606 USA Univ Toledo Toledo OH USA 43606 v Toledo, Dept Chem, Toledo, OH 43606 USA
Titolo Testata:
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
fascicolo: 10, volume: 26, anno: 2000,
pagine: 1059 - 1065
SICI:
0363-9045(2000)26:10<1059:AHLCAF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Keywords:
caffeine; HPLC; yohimbine HCl;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
5
Recensione:
Indirizzi per estratti:
Indirizzo: Alexander, KS Univ Toledo, Coll Pharm, 2801 W Bancroft St, Toledo, OH 43606 USA Univ Toledo 2801 W Bancroft St Toledo OH USA 43606 43606 USA
Citazione:
S. Mittal et al., "A high-performance liquid chromatography assay for yohimbine HCl analysis", DRUG DEV IN, 26(10), 2000, pp. 1059-1065

Abstract

The analysis used yohimbine HCl solution prepared from commercially available yohimbine HCl powder. Stability-indicating high-performance liquid chromatographic (HPLC) assay procedures were established and utilized to analyze the concentration of the drug. The method proved to be a simple model since it does not contain a buffer system. The mobile phase used, a methanol:water 70:30 ratio, was similar to that suggested by the manufacturer for thestorage of the column. Therefore, the solvent system saves analytical processing time since it does not require a change in the mobile phase before and after the analysis. The analytical method has been shown to be stabilityindicating. The assay method showed a retention time for yohimbine of 4.2 min; for caffeine, the internal standard, it was 2.3 min. The standard deviation and the coefficient of variation were under acceptable limits of 2% and were specifically 1.51% and 1.35% for within-day and between-day samples, respectively. The results showed that the degradation products obtained from stressing yohimbine HCl by heat and extremes in pH did not interfere with the yohimbine HCl peak, although the internal standard, caffeine, did show some interference due to having a retention time similar to the degradation products.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:18:02