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Titolo:
Bioenergetic targeting during organ preservation: P-31 magnetic resonance spectroscopy investigations into the use of fructose to sustain hepatic ATPturnover during cold hypoxia in porcine livers
Autore:
Changani, KK; Fuller, BJ; Bell, JD; Taylor-Robinson, S; Davidson, BR;
Indirizzi:
Royal Free & Univ Coll Med Sch, Dept Surg, London NW3 2QG, England Royal Free & Univ Coll Med Sch London England NW3 2QG n NW3 2QG, England Hammersmith Hosp, Imperial Coll Sch Med, MRC, Clin Sci Ctr,MR Unit, LondonW12 0NN, England Hammersmith Hosp London England W12 0NN ,MR Unit, LondonW12 0NN, England Hammersmith Hosp, Imperial Coll Sch Med, Div Med, London W12 0NN, England Hammersmith Hosp London England W12 0NN Div Med, London W12 0NN, England
Titolo Testata:
CRYOBIOLOGY
fascicolo: 1, volume: 41, anno: 2000,
pagine: 72 - 87
SICI:
0011-2240(200008)41:1<72:BTDOPP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER; GLUCOSE TRANSPORTER; ENERGY-METABOLISM; ISOLATED HEPATOCYTES; ADENINE-NUCLEOTIDES; MAMMALIAN LIVER; SMALL-INTESTINE; HYPOTHERMIA; INJURY; REGENERATION;
Keywords:
liver preservation; hypoxia; energy metabolism; P-31 NMR; fructose;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Fuller, BJ Royal Free & Univ Coll Med Sch, Dept Surg, Rowland Hill St, London NW3 2QG, England Royal Free & Univ Coll Med Sch Rowland Hill St LondonEngland NW3 2QG
Citazione:
K.K. Changani et al., "Bioenergetic targeting during organ preservation: P-31 magnetic resonance spectroscopy investigations into the use of fructose to sustain hepatic ATPturnover during cold hypoxia in porcine livers", CRYOBIOLOGY, 41(1), 2000, pp. 72-87

Abstract

During liver preservation, ATP supplies become depleted, leading to loss of cellular homeostatic controls and a cascade of ensuing harmful changes. Anaerobic glycolysis is unable to prolong ATP production for a significant period because of metabolic blockade. Our aim was to promote glycolysis during liver cold hypoxia by supplying fructose as an additional substrate, compared to supplementation with an equivalent concentration of glucose. Porcine livers (two groups; n = 5 in each) were retrieved by clinical harvestingtechniques and subjected to two cycles of cold hypoxia and oxygenated hypothermic reperfusion. In the second cycle of reperfusion, the perfusate was supplemented with either 10 mmol/L glucose (Group I)or 10 mmol/L fructose (Group 2). During reperfusion in both groups, similar levels of ATP were detected by phosphorus magnetic resonance spectroscopy (P-31 MRS). However, during subsequent hypoxia, ATP was detected for much longer periods in the fructose-perfused group. The rate of ATP loss was sevenfold slower during hypoxia in the presence of fructose than in the presence of glucose (ATP consumption of -7.2 x 10 % total P-31 for Group 1 versus -1.0 x 10(-3)% total P-31 for Group 2: P < 0.001). The changes in ATP were mirrored by differencesin other MRS-detectable intermediates: e.g., inorganic phosphate was significantly higher during subsequent hypoxia in Group 1 (45.7 +/- 2.7% total P-31) than in Group 2 (33.7 +/- 1.1% total P-31: P < 0.01). High-resolution MRS of liver tissue extracts demonstrated that fructose was metabolized mainly via fructose 1-phosphate. We conclude that fructose supplied by brief hypothermic perfusion may improve the bioenergeric status of cold hypoxic livers by sustaining anaerobic glycolysis via a point of entry into the pathway that is different from that for glucose. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 14:40:35