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Titolo:
Influence of age and HLA type on interferon-gamma (IFN-gamma) responses toa naturally occurring polymorphic epitope of Plasmodium falciparum liver stage antigen-1 (LSA-1)
Autore:
Bucci, K; Kastens, W; Hollingdale, MR; Shankar, A; Alpers, MP; King, CL; Kazura, JW;
Indirizzi:
Case Western Reserve Univ, Sch Med, Div Geog Med, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Univ Leeds, Dept Biol, Leeds, W Yorkshire, England Univ Leeds Leeds W Yorkshire England t Biol, Leeds, W Yorkshire, England Papua New Guinea Inst Med Res, Goroka, Papua N Guinea Papua New Guinea Inst Med Res Goroka Papua N Guinea oka, Papua N Guinea Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Int Hlth, Baltimore, MD USA Johns Hopkins Univ Baltimore MD USA th, Dept Int Hlth, Baltimore, MD USA Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD USA Johns Hopkins Univ Baltimore MD USA crobiol & Immunol, Baltimore, MD USA
Titolo Testata:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
fascicolo: 1, volume: 122, anno: 2000,
pagine: 94 - 100
SICI:
0009-9104(200010)122:1<94:IOAAHT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL RESPONSES; PAPUA-NEW-GUINEA; EAST SEPIK PROVINCE; CIRCUMSPOROZOITE PROTEIN; VACCINE TRIALS; WOSERA AREA; CLASS-I; MALARIA; IMMUNITY; RESISTANCE;
Keywords:
malaria; HLA; LSA-1; polymorphism; cytokine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kazura, JW Case Western Reserve Univ, Sch Med, Div Geog Med, Room W137,2109 Adelbert Rd, Cleveland, OH 44106 USA Case Western Reserve Univ Room W137,2109 Adelbert Rd Cleveland OH USA 44106
Citazione:
K. Bucci et al., "Influence of age and HLA type on interferon-gamma (IFN-gamma) responses toa naturally occurring polymorphic epitope of Plasmodium falciparum liver stage antigen-1 (LSA-1)", CLIN EXP IM, 122(1), 2000, pp. 94-100

Abstract

Antigenic polymorphism and HLA restriction may limit the immunogenicity ofa subunit vaccine against liver-stage Plasmodium falciparum. We examined 59 clinical isolates and five laboratory clones of P, falciparum for polymorphism in the N- and C-terminal regions of LSA-1, evaluated binding of the corresponding peptides to selected HLA class I alleles, and measured IFN-gamma responses in residents of a malaria-endemic area of Papua New Guinea where HLA-A*1101, -24, -B13, and -B40 are the most common class I alleles. LSA-1 polymorphism was limited to a single non-synonymous mutation encoding serine (S), proline (P), or threonine (T) at amino acid 85, Nine-mer 84-92 peptides with S, T, or P at the primary anchor position bound differentially to HLA-A11, -A2, and -B7. IFN-gamma ELISPOT responses increased with age inmalaria-exposed subjects: 14-16% and 30-36% of 2-5- and 6-54-year-olds, respectively, had greater than or equal to 10 IFN-gamma-secreting cells/10(6)peripheral blood mononuclear cells when stimulated with at least one peptide variant (P < 0.05). IFN-gamma responses to all three peptides were also greater for older than younger individuals. No children < 3 years old had lymphocytes that responded to all three 84-92 peptides, whereas 45% of adults (mean age 45 years) had aggregated IFN-gamma responses. These data support the notion that age-related cumulative exposure to P. falciparum increases the frequency of IFN-gamma responses to polymorphic epitopes of liver-stage antigens such as LSA-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/10/20 alle ore 15:37:41