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Titolo:
Peroxisome proliferator-activated receptor activators inhibit lipopolysaccharide-induced turner necrosis factor-alpha expression in neonatal rat cardiac myocytes
Autore:
Takano, H; Nagai, T; Asakawa, M; Toyozaki, T; Oka, T; Komuro, I; Saito, T; Masuda, Y;
Indirizzi:
Chiba Univ, Sch Med, Dept Internal Med 3, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 rnal Med 3, Chuo Ku, Chiba 2608670, Japan Univ Tokyo, Grad Sch Med, Dept Cardiovasc Med, Tokyo, Japan Univ Tokyo Tokyo Japan Grad Sch Med, Dept Cardiovasc Med, Tokyo, Japan
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 7, volume: 87, anno: 2000,
pagine: 596 - 602
SICI:
0009-7330(20000929)87:7<596:PPRAIL>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CREB-BINDING PROTEIN; SMOOTH-MUSCLE CELLS; PPAR-GAMMA; KAPPA-B; TISSUE DISTRIBUTION; ENDOTHELIAL-CELLS; TRANSGENIC MICE; MAMMALIAN HEART; CYTOKINES; CARDIOMYOPATHY;
Keywords:
peroxisome proliferator-activated receptor; tumor necrosis factor-alpha; nuclear factor-kappa B; cardiac myocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Takano, H Chiba Univ, Sch Med, Dept Internal Med 3, Chuo Ku, 1-8-1 Inohana, Chiba 2608670, Japan Chiba Univ 1-8-1 Inohana Chiba Japan 2608670 iba 2608670, Japan
Citazione:
H. Takano et al., "Peroxisome proliferator-activated receptor activators inhibit lipopolysaccharide-induced turner necrosis factor-alpha expression in neonatal rat cardiac myocytes", CIRCUL RES, 87(7), 2000, pp. 596-602

Abstract

Peroxisome proliferator-activated receptors (PPARs) are transcription factors belonging to the nuclear receptor superfamily. Recently, PPAR activators have been shown to inhibit the production of proinflammatory cytokines inmacrophages or vascular smooth muscle cells. It has been reported that tumor necrosis factor-alpha (TNF-alpha) expression is elevated in the failing heart and that TNF-alpha has a negative inotropic effect on cardiac myocytes. Therefore, we examined the effects of PPAR alpha and PPAR gamma activators on expression of TNF-alpha in neonatal rat cardiac myocytes. Northern blot analysis revealed expression of PPAR alpha and PPAR gamma mRNA in cardiac myocytes. Immunofluorescent staining demonstrated that both PPAR alpha and PPAR gamma were expressed in the nuclei of cells. When cardiac myocytes were transfected with PPAR responsive element (PPRE)-luciferase reporter plasmid, both PPAR alpha and PPAR gamma activators increased the promoter activity. Cardiomyocytes were stimulated with Lipopolysaccharide (LPS), and thelevels of TNF-alpha in the medium were measured by ELISA. After exposure to LPS, the levels of TNF-alpha significantly increased. However, pretreatment of myocytes with PPAR alpha or PPAR gamma activators decreased LPS-induced expression of TNF-alpha in the medium. Both PPAR alpha and PPAR gamma activators also inhibited LPS-induced increase in TNF-alpha mRNA in myocytes. In addition, electrophoretic mobility shift assays demonstrated that PPAR activators reduced LPS-induced nuclear factor-kappa B activation. These results suggest that both PPAR alpha and PPAR gamma activators inhibit cardiacexpression of TNF-alpha in part by antagonizing nuclear factor-kappa B activity and that treatment with PPAR activators may lead to improvement in congestive heart failure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:45:27