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Titolo:
Co-transfection of MRP and bcl-2 antisense S-oligodeoxynucleotides reducesdrug resistance in cisplatin-resistant lung cancer cells
Autore:
Wang, J; Liu, XY; Jiang, W;
Indirizzi:
Beijing Med Univ, Sch Oncol, Dept Internal Med, Beijing 100036, Peoples R China Beijing Med Univ Beijing Peoples R China 100036 100036, Peoples R China
Titolo Testata:
CHINESE MEDICAL JOURNAL
fascicolo: 10, volume: 113, anno: 2000,
pagine: 957 - 960
SICI:
0366-6999(200010)113:10<957:COMABA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN MRP; OLIGONUCLEOTIDES; INHIBITION; EXPRESSION; APOPTOSIS;
Keywords:
A(549) and A(549)(DDP) cell lines; drug resistance; apoptosis; lung neoplasms; antisense; S-oligodeoxynucleotide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, J Beijing Med Univ, Sch Oncol, Dept Internal Med, Beijing 100036, Peoples R China Beijing Med Univ Beijing Peoples R China 100036 Peoples R China
Citazione:
J. Wang et al., "Co-transfection of MRP and bcl-2 antisense S-oligodeoxynucleotides reducesdrug resistance in cisplatin-resistant lung cancer cells", CHIN MED J, 113(10), 2000, pp. 957-960

Abstract

Objective To detect the influence of antisense s-oligodeoxynucleotides (S-ODNs) of bd-2 and multidrug resistance-associated protein (MRP) genes multidrug resistance-associated protein gene and bcl-2 antisense S-oligodeoxynucleotides on cisplatin-resistant lung adenocarcinoma cell line A(549)(DDP) which overexpresses both bcl-2 and MRP. Methods A549DDP cells were treated with sense and antisense S-ODN mediatedby lipofection. Expression of MRP and bcl-2 mRNA and protein in the treated cells was measured by RT-PCR and flow cytometry (FCM), respectively. Apoptosis was identified by DNA electrophoresis and terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP nick end-labeling(TUNEL). The degree of drug resistance of the treated cells was detected by a cell viability 3'-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-tefrazolium bromide thiazolylblue (MTT) assay. Results Expression of bcl-2 and MRP significantly decreased in the cells treated with bcl-2 or/and MRP antisense S-ODN for 48h as compared to the cells untreated and sense-treated (P < 0.05). Resistance to cisplatin in the cells treated with bcl-2 or/and MRP antisense S-ODN decreased by 60.6% (6.5 times), 56.4% (7.2 times) and 71.0% (4.8 times), respectively, which paralleled the decrease of bcl-2 and MRP expression. Similarly, the resistance toetoposide and epirubicin in antisense-treated cells also reduced in parallel to decreases of the two gene expressions. The drug resistance in sense-treated cells was similar to that in untreated cells. Statistically significant dose-and concentration-dependent increases of apoptotic cells were observed in the groups exposed to 100 mu mol/L cisplatin for 48 h after treatment by bcl-2 or/and MRP antisense. Conclusion Bcl-2 and MRP were at least additive and possibly synergistic in conferring drug resistance in a cisplatin-resistant lung adenocarcinoma cell line. Antisense S-ODN could attenuate drug resistance by promoting cells apoptosis, which might lead to a new treatment for patients with non-small cell lung cancers (NSCLCs) who are refractory to conventional chemotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:07:42