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Titolo:
Osmoprotective effect of glycine betaine on thrombopoietin production in hyperosmotic Chinese hamster ovary cell culture: Clonal variations
Autore:
Kim, TK; Ryu, JS; Chung, JY; Kim, MS; Lee, GM;
Indirizzi:
Korea Adv Inst Sci & Technol, Dept Biol Sci, Yusong Gu, Taejon 305701, South Korea Korea Adv Inst Sci & Technol Taejon South Korea 305701 5701, South Korea
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 16, anno: 2000,
pagine: 775 - 781
SICI:
8756-7938(200009/10)16:5<775:OEOGBO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY PRODUCTION; HYBRIDOMA CELLS; PRODUCTION KINETICS; OSMOTIC-PRESSURE; SODIUM-BUTYRATE; STRESS; GROWTH; EXPRESSION; EVOLUTION; TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, GM Korea Adv Inst Sci & Technol, Dept Biol Sci, Yusong Gu, Kusong Dong 373-1,Taejon 305701, South Korea Korea Adv Inst Sci & Technol Kusong Dong373-1 Taejon South Korea 305701
Citazione:
T.K. Kim et al., "Osmoprotective effect of glycine betaine on thrombopoietin production in hyperosmotic Chinese hamster ovary cell culture: Clonal variations", BIOTECH PR, 16(5), 2000, pp. 775-781

Abstract

When 23 recombinant Chinese hamster ovary (rCHO) cell clones were cultivated in hyperosmolar medium resulting from NaCl addition (533 mOsm/kg), theirspecific thrombopoietin (TPO) productivity (q(TPO)) was increased. However, due to depressed cell growth at elevated osmolality, no enhancement in the maximum TPO titer was made in batch cultures of all 23 clones. To test the feasibility of using glycine betaine, known as a strong osmoprotective compound, for improved TPO production in hyperosmotic rCHO cell cultures, hyperosmotic batch cultures of 23 clones were performed in the presence of 15 mM glycine betaine. Glycine betaine was found to have a strong osmoprotective effect on all 23 clones. Inclusion of 15 mM glycine betaine in hyperosmolar medium enabled 22 clones to grow at 542 mOsm/kg, where most clones could not grow in the absence of glycine betaine, but at a cost of reduced qTPOHowever, the relative decrease in q(TPO) varied significantly among clones. Thus, efficacy of the simultaneous use of hyperosmotic pressure and glycine betaine as a means to improve foreign protein production was variable among clones. Six out of 23 clones displayed more than a 40% increase in the maximum TPO titer in the hyperosmolar medium containing glycine betaine, compared with that in the standard medium with a physiological osmolality. Taken together, the results obtained here emphasize the importance of selection of clones for the successful use of hyperosmotic pressure and glycine betaine as an economical means to improve TPO production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:33:32