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Titolo:
An update of pTRIDENT multicistronic expression vectors: pTRIDENTs containing novel streptogramin-responsive promoters
Autore:
Moser, S; Schlatter, S; Fux, C; Rimann, M; Bailey, JE; Fussenegger, M;
Indirizzi:
ETH Zurich, Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 chnol, CH-8093 Zurich, Switzerland
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 16, anno: 2000,
pagine: 724 - 735
SICI:
8756-7938(200009/10)16:5<724:AUOPME>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN-FLUORESCENT PROTEIN; IMMEDIATE-EARLY GENE; HAMSTER OVARY CELLS; MULTIDRUG-RESISTANCE GENE; HUMAN CYTOMEGALO-VIRUS; MAMMALIAN-CELLS; TRANSGENIC MICE; ESCHERICHIA-COLI; PEF-BOS; REPORTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Fussenegger, M ETH Zurich, Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 Zurich, Switzerland
Citazione:
S. Moser et al., "An update of pTRIDENT multicistronic expression vectors: pTRIDENTs containing novel streptogramin-responsive promoters", BIOTECH PR, 16(5), 2000, pp. 724-735

Abstract

We present an update on the pTRIDENT multicistronic mammalian expression vectors and their implications in various metabolic engineering and therapeutic applications. The pTRIDENT vector family has been expanded by construction of a new set of pTRPDENT-based vectors containing constitutive promoters of human origin (ubiquitin C and EF-1 alpha promoters) and selectable markers (zeocin resistance) and expressing different reporter genes (secreted alkaline phosphatase (SEAP) and the secreted single-chain urokinase-type plasminogen activator (low-M-r u-PA)). In addition, we have constructed pTRIDENT derivatives with novel streptogramin-repressible and streptogramin-inducible promoters for simultaneous and adjustable expression of three different transgenes. Streptogramin-inducible and tetracycline-repressible pTRIDENT derivatives were used to simultaneously control expression of three fluorescent proteins in mammalian cells: the enhanced cyan fluorescent protein (CFP), the recently isolated red fluorescent protein (RFP, also designated dsRed), and the enhanced yellow fluorescent protein (YFP). Owing to their modular structure, the pTRIDENT vector family represents a construction kit for the design of novel multicistronic expression constructs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:54:15