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Titolo:
Decreased cellular activity and replicative capacity of osteoblastic cellsisolated from the periarticular bone of rheumatoid arthritis patients compared with osteoarthritis patients
Autore:
Yudoh, K; Matsuno, H; Osada, R; Nakazawa, F; Katayama, R; Kimura, T;
Indirizzi:
Toyama Med & Pharmaceut Univ, Dept Orthopaed Surg, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ Toyama Japan 9300194 Toyama 9300194, Japan
Titolo Testata:
ARTHRITIS AND RHEUMATISM
fascicolo: 10, volume: 43, anno: 2000,
pagine: 2178 - 2188
SICI:
0004-3591(200010)43:10<2178:DCAARC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TELOMERASE ACTIVITY; HUMAN FIBROBLASTS; LIFE-SPAN; OSTEOCLAST DIFFERENTIATION; IMMORTAL CELLS; IN-VITRO; AGE; METHOTREXATE; OSTEOPOROSIS; LEUKOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Yudoh, K Toyama Med & Pharmaceut Univ, Dept Orthopaed Surg, 2630 Sugitani,Toyama 9300194, Japan Toyama Med & Pharmaceut Univ 2630 Sugitani Toyama Japan 9300194
Citazione:
K. Yudoh et al., "Decreased cellular activity and replicative capacity of osteoblastic cellsisolated from the periarticular bone of rheumatoid arthritis patients compared with osteoarthritis patients", ARTH RHEUM, 43(10), 2000, pp. 2178-2188

Abstract

Objective. Periarticular osteopenia is frequently observed in rheumatoid arthritis (RA), Bone loss has been considered to be at least partly due to inadequate bone formation, which in turn, is largely dependent on the numberof osteoblasts and the osteoblastic activity. Normal human somatic cells undergo a finite number of cell divisions and ultimately enter a nondividingstate called replicative senescence. It has been proposed that the telomere, the terminal sequence of chromosomes, is the mitotic clock that triggerssenescence. In the present study, we sought to clarify the relationship between periarticular osteopenia and osteoblast replicative senescence in RA,Methods, We examined age-related changes in cellular activity (alkaline phosphatase activity, osteocalcin and C-terminal type I procollagen secretion, and cAMP response to parathyroid hormone), replicative capacity, and senescent cell expression in osteoblasts from periarticular bone samples obtained from 15 patients with RA and 15 age-matched patients with osteoarthritis(OA), Cellular replicative capacity was analyzed by the mean telomere length and in vitro remaining replicative lifespan of the cells. Results. In both OA and RA groups, the cell proliferation rate, the levelsof osteoblastic markers, mean telomere length, and replicative lifespan inosteoblastic cells gradually decreased with the increasing age of the donor. The percentage of senescent osteoblastic cells in the periarticular boneincreased with age in both groups, and the rate of expression of senescentcells was higher in RA patients than in age-matched OA patients. The osteoblastic activities and replicative capacity of osteoblastic cells from RA patients were lower than those from OA patients at any donor age. The age-related decreases in the osteoblastic activity and replicative capacity of osteoblastic cells from periarticular bone were greater in RA patients than in OA patients. Conclusion. Our results suggest that osteoblast replicative senescence in periarticular bones occurs more rapidly with aging in BA than in OA patients and contributes to periarticular osteopenia in RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:03:18