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Titolo:
Substrate flexibility regulates growth and apoptosis of normal but not transformed cells
Autore:
Wang, HB; Dembo, M; Wang, YL;
Indirizzi:
Univ Massachusetts, Sch Med, Dept Physiol, Worcester, MA 01605 USA Univ Massachusetts Worcester MA USA 01605 hysiol, Worcester, MA 01605 USA Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA Boston Univ Boston MAUSA 02215 v, Dept Biomed Engn, Boston, MA 02215 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 5, volume: 279, anno: 2000,
pagine: C1345 - C1350
SICI:
0363-6143(200011)279:5<C1345:SFRGAA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASES; FOCAL ADHESIONS; CYCLIN D1; SHAPE; FIBROBLASTS; LOCOMOTION; MECHANOTRANSDUCTION; INTEGRINS; RELEASE; TENSION;
Keywords:
mechanical signaling; cell cycle; cell shape; traction force; cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, YL Univ Massachusetts, Sch Med, Dept Physiol, 377 Plantat St,Rm 327,Worcester, MA 01605 USA Univ Massachusetts 377 Plantat St,Rm 327 WorcesterMA USA 01605 A
Citazione:
H.B. Wang et al., "Substrate flexibility regulates growth and apoptosis of normal but not transformed cells", AM J P-CELL, 279(5), 2000, pp. C1345-C1350

Abstract

One of the hallmarks of oncogenic transformation is anchorage-independent growth (27). Here we demonstrate that responses to substrate rigidity play a major role in distinguishing the growth behavior of normal cells from that of transformed cells. We cultured normal or H-ras-transformed NIH 3T3 cells on flexible collagen-coated polyacrylamide substrates with similar chemical properties but different rigidity. Compared with cells cultured on stiff substrates, nontransformed cells on flexible substrates showed a decreasein the rate of DNA synthesis and an increase in the rate of apoptosis. These responses on flexible substrates are coupled to decreases in cell spreading area and traction forces. In contrast, transformed cells maintained their growth and apoptotic characteristics regardless of substrate flexibility. The responses in cell spreading area and traction forces to substrate flexibility were similarly diminished. Our results suggest that normal cells are capable of probing substrate rigidity and that proper mechanical feedback is required for regulating cell shape, cell growth, and survival. The loss of this response can explain the unregulated growth of transformed cells.

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Documento generato il 18/01/21 alle ore 14:37:20