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Titolo:
Sequence and functional analysis of a homolog of interleukin-10 encoded bythe Parapoxvirus orf virus
Autore:
Fleming, SB; Haig, DM; Nettleton, P; Reid, HW; McCaughan, CA; Wise, LM; Mercer, AA;
Indirizzi:
Hlth Res Council New Zealand, Virus Res Unit, Dunedin, New Zealand Hlth Res Council New Zealand Dunedin New Zealand , Dunedin, New Zealand Univ Otago, Ctr Gene Res, Dunedin, New Zealand Univ Otago Dunedin New Zealand tago, Ctr Gene Res, Dunedin, New Zealand Int Res Ctr, Moredun Res Inst, Penicuik EH26 0PZ, Midlothian, Scotland IntRes Ctr Penicuik Midlothian Scotland EH26 0PZ Z, Midlothian, Scotland
Titolo Testata:
VIRUS GENES
fascicolo: 1-2, volume: 21, anno: 2000,
pagine: 85 - 95
SICI:
0920-8569(200008)21:1-2<85:SAFAOA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN INTERFERON-GAMMA; EQUINE HERPESVIRUS TYPE-2; ENDOTHELIAL GROWTH-FACTOR; DENDRITIC CELLS; CRYSTAL-STRUCTURE; VACCINIA VIRUS; CONTAGIOUS ECTHYMA; CYTOKINE RESPONSE; INFECTION; GENE;
Keywords:
orf virus; poxvirus; interleukin-10; gene function; sequence homology; evolutionary relationships; genetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Fleming, SB Hlth Res Council New Zealand, Virus Res Unit, Dunedin, New Zealand Hlth Res Council New Zealand Dunedin New Zealand ew Zealand
Citazione:
S.B. Fleming et al., "Sequence and functional analysis of a homolog of interleukin-10 encoded bythe Parapoxvirus orf virus", VIRUS GENES, 21(1-2), 2000, pp. 85-95

Abstract

Orf virus isa large DNA virus and is the type species of the Parapoxvirus genus of the family Poxviridae. Orf virus infects the epithelium of sheep and goats and is transmissible to humans. Recently we discovered a gene in orf virus that encodes a polypeptide with remarkable homology to mammalian interleukin (IL-10) and viral encoded IL-10s of herpes viruses. The predicted polypeptide sequence shows high levels of amino acid identity to IL-10 ofsheep (80%), cattle (75%), humans (67%) and mice (64%), as well as IL-10-like proteins of Epstein-Barr virus (63%) and equine herpes; virus (67%). The C-terminal region, comprising two-thirds of the orf virus protein, is identical to ovine IL-10 which suggests that this gene has been captured from its host sheep during the evolution of orf virus. In contrast the N-terminal region shows little homology with cellular IL-10s and in this respect resembles other viral IL-10s. IL-10s is a pleiotrophic cytokine that can exerteither immunostimulatory or immunosuppressive effects on many cell types. IL-10 is a potent anti-inflammatory cytokine with inhibitory effects on non-specific immunity in particular macrophage function and Th1 effector function. Our studies so far, indicate, that the functional activities of orf virus IL-10 are the same as ovine IL-I10 Orf virus IL-10 stimulates mouse thymocyte proliferation and inhibits cytokine synthesis in lipopolysaccharide-activated ovine macrophages, peripheral blood monocytes and keratinocytes. Infection of sheep with an IL-10 deletion mutant of orf virus has shown that interferon-gamma levels are higher in tissue infected with the mutant virus than the parent virus. The functional activities of IL-10 and our data on orf virus IL-10 suggest a role in immune evasion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:14:47