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Titolo:
Rapid detection of porcine reproductive and respiratory syndrome viral nucleic acid in blood using a fluorimeter based PCR method
Autore:
Spagnuolo-Weaver, M; Walker, IW; Campbell, ST; Mc Neilly, F; Adair, BM; Allan, GM;
Indirizzi:
Vet Sci Div, Dept Virol, Belfast BT4 3SD, Antrim, North Ireland Vet Sci Div Belfast Antrim North Ireland BT4 3SD D, Antrim, North Ireland Queens Univ Belfast, Dept Vet Sci, Belfast BT4 3SD, Antrim, North Ireland Queens Univ Belfast Belfast Antrim North Ireland BT4 3SD m, North Ireland
Titolo Testata:
VETERINARY MICROBIOLOGY
fascicolo: 1, volume: 76, anno: 2000,
pagine: 15 - 23
SICI:
0378-1135(20000915)76:1<15:RDOPRA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; SYNDROME PRRS VIRUS; HUMAN-IMMUNODEFICIENCY-VIRUS; REVERSE TRANSCRIPTION; FILTER-PAPER; AMPLIFICATION; DNA; DIFFERENTIATION; SPOTS;
Keywords:
porcine reproductive and respiratory syndrome virus (PRRSV); pig-viruses; diagnosis-viruses; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Allan, GM Vet Sci Div, Dept Virol, Belfast BT4 3SD, Antrim, North Ireland Vet Sci Div Belfast Antrim North Ireland BT4 3SD North Ireland
Citazione:
M. Spagnuolo-Weaver et al., "Rapid detection of porcine reproductive and respiratory syndrome viral nucleic acid in blood using a fluorimeter based PCR method", VET MICROB, 76(1), 2000, pp. 15-23

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus recognised world wide as an important cause of reproductive failure andpneumonia in pigs. American and European strains of PRRSV, differentiated antigenically and genomically, have been reported. PRRSV infections are currently diagnosed using serology, virus isolation and/or immunocytochemistry. In order to overcome various drawbacks associated with these techniques, conventional, block-based RT-PCR methods for the detection of PRRSV nucleicacid in clinical samples have been described. These methods require gel electrophoresis for analysis of PCR products and present high risk of DNA carry-over contamination between the samples tested. We describe the detectionof PRRSV RNA in serum samples and in blood impregnated filter disks (FDs),obtained from experimentally inoculated pigs, using a closed-tube, fluorimeter-based PCR assay. The assay eliminates the use of gel electrophoresis, and is as sensitive and specific as the conventional block-based PCR assay,detecting positive samples as early as 1 day post-inoculation. We also report a rapid fluorimeter based PCR method for differentiating American and European strains of PRRSV. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 12:39:27