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Titolo:
NIH 3T3 cells or engineered NIH 3T3 cells stably expressing GDNF can protect primary dopaminergic neurons
Autore:
Ma, DD; Wang, XM; Han, JS;
Indirizzi:
Beijing Med Univ, Ctr Res Neurosci, Beijing 100083, Peoples R China Beijing Med Univ Beijing Peoples R China 100083 100083, Peoples R China
Titolo Testata:
NEUROLOGICAL RESEARCH
fascicolo: 6, volume: 22, anno: 2000,
pagine: 538 - 544
SICI:
0161-6412(200009)22:6<538:N3COEN>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTROPHIC FACTOR GDNF; PARKINSONS-DISEASE; IN-VIVO; MESSENGER-RNA; RAT MODEL; OXIDATIVE STRESS; FACTOR PROMOTES; MOTOR-NEURONS; TOXICITY; SYSTEM;
Keywords:
GDNF; Parkinson's disease; NIH 3T3 cells; dopaminergic neurons; engineered cells; tyrosine hydroxylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Ma, DD Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, Box 8069,600 S Euclid Ave, St Louis, MO 63110 USA Washington Univ Box 8069,600 S Euclid Ave St Louis MO USA 63110 USA
Citazione:
D.D. Ma et al., "NIH 3T3 cells or engineered NIH 3T3 cells stably expressing GDNF can protect primary dopaminergic neurons", NEUROL RES, 22(6), 2000, pp. 538-544

Abstract

Glial cell line-derived neurotrophic factor (GDNF) shows potent and relatively specific protective effects on dopaminergic neurons. However, the sizeof the GDNF protein (MW 32-42 kDa) precludes the clinical use of GDNF via parenteral administration. It would thus be useful to have a cell line thatstably secretes GDNF with full biological activities. The present study shows that NIH 3T3 cells express a considerable amount of GDNF. Alter co-culturing with primary E14-E16 midbrain neurons, such cells protected primary rat midbrain TH-immunopositive neurons from degeneration and MPP+ toxicity. In order to enhance endogenous GDNF expression, NIH 3T3 cells were stably transfected with GDNF cDNA with the Kozak sequence. The clones with the highest GDNF expression level were selected. The protective effects of engineered cells increased as the GDNF expression level increased. These cell linesmay merit clinical investigation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 23:56:55