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Titolo:
Transposition of maize Ac/Ds transposable elements in the yeast Saccharomyces cerevisiae
Autore:
Weil, CF; Kunze, R;
Indirizzi:
Univ Idaho, Dept Biol Sci, Moscow, ID 83843 USA Univ Idaho Moscow ID USA 83843 Idaho, Dept Biol Sci, Moscow, ID 83843 USA Univ Munich, Inst Genet & Mikrobiol, D-8000 Munich, Germany Univ Munich Munich Germany D-8000 et & Mikrobiol, D-8000 Munich, Germany
Titolo Testata:
NATURE GENETICS
fascicolo: 2, volume: 26, anno: 2000,
pagine: 187 - 190
SICI:
1061-4036(200010)26:2<187:TOMATE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATOR AC TRANSPOSASE; DNA TRANSPOSITION; IN-VIVO; EXCISION; EXPRESSION; GENE; ARABIDOPSIS; TOBACCO; RECOMBINATION; DISSOCIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kunze, R Univ Cologne, Bot Inst 2, Cologne, Germany Univ Cologne CologneGermany gne, Bot Inst 2, Cologne, Germany
Citazione:
C.F. Weil e R. Kunze, "Transposition of maize Ac/Ds transposable elements in the yeast Saccharomyces cerevisiae", NAT GENET, 26(2), 2000, pp. 187-190

Abstract

Excision by transposons is associated with chromosome breaks; generally, host-cell proteins repair this damage, often introducing mutations. Many transposons also use host proteins in the transposition mechanism or in regulation(1-4). Transposition in systems lacking host factors that influence thebehaviour of these transpositions is useful in determining what those factors are and how they work. In addition, features of transposition and regulation intrinsic to the element itself can be determined. Maize Activator/Dissociation (Ac/Ds) elements transpose in a wide variety of heterologous plants, but their characteristics in these other systems differ from those in maize, including their response to increasing genetic dosage(5,6) and the types of repair products recovered following excision(7). Two Arabidopsis thaliana mutants (iae1 and iae2) show increased Ac transposition frequencies(8). These mutants, and the differences mentioned above, suggest the involvement of host proteins in Ac/Ds activity and potential differences between these proteins among plant species. Here we report that Ac/Ds elements, members of the hAT (hobo, Ac, Tam3) superfamily, transpose in the yeast Saccharomyces cerevisiae, an organism lacking class II ('cut and paste') transposons. This demonstrates that plant-specific proteins are not essential for Ac/Ds transposition. The yeast system is valuable for dissecting the Ac/Ds transposition mechanism and identifying host factors that can influence transposition and the repair of DNA damage induced by Ac/Ds. Mutations caused byDs excision in yeast suggest formation of a DNA-hairpin intermediate, and reinsertions occur throughout the genome with a frequency similar to that in plants. The high proportion of Ac/Ds reinsertions also makes this system an in vivo mutagenesis and reverse genetics tool in yeast and, presumably, other eukaryotic systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:32:39