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Titolo:
Dexamethasone stimulates ribosomal protein L32 gene transcription in rat myoblasts
Autore:
Sienna, N; Larson, DE; Sells, BH;
Indirizzi:
Univ Guelph, Dept Mol Biol & Genet, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 & Genet, Guelph, ON N1G 2W1, Canada
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 167, anno: 2000,
pagine: 127 - 137
SICI:
0303-7207(20000925)167:1-2<127:DSRPLG>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN GLUCOCORTICOID RECEPTOR; HORMONE MESSENGER-RNA; LYMPHOSARCOMA CELLS; NUCLEAR RECEPTORS; SACCHAROMYCES-CEREVISIAE; TRANSACTIVATION DOMAIN; FACTOR EXPRESSION; BINDING-PROTEIN; POLYMERASE-II; DNA-BINDING;
Keywords:
ribosomal protein gene expression; myoblasts; dexamethasone; RU38486;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Sells, BH Univ Guelph, Dept Mol Biol & Genet, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 Guelph, ON N1G 2W1, Canada
Citazione:
N. Sienna et al., "Dexamethasone stimulates ribosomal protein L32 gene transcription in rat myoblasts", MOL C ENDOC, 167(1-2), 2000, pp. 127-137

Abstract

Incubation of rat L6 myoblasts for 24 h with 10(-7) M dexamethasone, a glucocorticoid analogue, resulted in a 2.5-fold increase in the rate of ribosomal protein L32 (rpL32) gene transcription with a corresponding increase inthe level of rpL32 mRNA. The increased rate of transcription was accompanied by a dramatic enhancement in binding of the delta, but not beta and gamma, factors to the rpL32 gene promoter ai measured by gel mobility shift assays. This increased: binding reflects a change in the activity of the deltafactor since its level is unchanged by dexamethasone treatment. The presence of the glucocorticoid analogue RU38486 reversed the stimulating effect of dexamethasone on rpL32 gene transcription and binding of the delta factorto the delta element. These results suggest that the mechanism which enhances rpL32 gene transcription in dexamethasone-treated rat L6 myoblasts involves glucocorticoid-receptor mediated changes in the activity of the delta factor. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:48:30