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Titolo:
Elastic fiber proteins in the glomerular mesangium in vivo and in cell culture
Autore:
Sterzel, RB; Hartner, A; Schlotzer-Schrehardt, U; Voit, S; Hausknecht, B; Doliana, R; Colombatti, A; Gibson, MA; Braghetta, P; Bressan, GM;
Indirizzi:
Univ Erlangen Nurnberg, Med Klin 4, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Univ Erlangen Nurnberg, Augenklin Poliklin, D-91054 Erlangen, Germany UnivErlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Inst Nazl Tumori, Ctr Riferimento Oncol, Aviano, Italy Inst Nazl Tumori Aviano Italy ori, Ctr Riferimento Oncol, Aviano, Italy Univ Udine, Dipartimento Sci & Tecnol Biomed, I-33100 Udine, Italy Univ Udine Udine Italy I-33100 Sci & Tecnol Biomed, I-33100 Udine, Italy Univ Adelaide, Dept Pathol, Adelaide, SA, Australia Univ Adelaide Adelaide SA Australia Dept Pathol, Adelaide, SA, Australia Univ Padua, Inst Istol & Embriol, Padua, Italy Univ Padua Padua ItalyUniv Padua, Inst Istol & Embriol, Padua, Italy
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 4, volume: 58, anno: 2000,
pagine: 1588 - 1602
SICI:
0085-2538(200010)58:4<1588:EFPITG>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; FIBRILLIN-CONTAINING MICROFIBRILS; EXTRACELLULAR-MATRIX; BINDING-PROTEIN; MARFAN-SYNDROME; MESSENGER-RNA; RAT-KIDNEY; EXPRESSION; INTEGRIN; LOCALIZATION;
Keywords:
hydrostatic pressure; cell elasticity; renal vasculature; extracellular matrix; growth factor; glomerular capillary tuft; transforming growth factor-beta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Sterzel, RB Univ Erlangen Nurnberg, Med Klin 4, Krankenhausstr 12, D-91054Erlangen, Germany Univ Erlangen Nurnberg Krankenhausstr 12 Erlangen Germany D-91054
Citazione:
R.B. Sterzel et al., "Elastic fiber proteins in the glomerular mesangium in vivo and in cell culture", KIDNEY INT, 58(4), 2000, pp. 1588-1602

Abstract

Background. Glomerular capillaries of the mammalian kidney are exposed to high intraluminal hydrostatic pressures and require elastic constraint to maintain size, shape, and integrity. previous morphological and functional studies indicated that the extracellular matrices of glomeruli, that is, basement membrane and mesangial matrix, contribute to glomerular resilience and mechanical stability. Immunofluorescence microscopy findings demonstratedelastic fiber components to be located in the renal vasculature. includingglomeruli. The aim of this study was to clarify the exact glomerular localization, composition, and cellular production of these proteins. Methods. We examined the renal distribution of the elastic fiber proteins fibrillin-1. emilin. microfibril-associated glycoproteins (MAGPs) 1 and 2, latent transforming growth factor-binding protein-1 (LTBP-1), and elastin using immunohistology and immunoelectron microscopy of human, rat, and mousekidneys. In mesangial cell cultures, we also studied the expression and extracellular deposition of such proteins by use of Northern blotting and immunocytochemistry. Results. Fibrillin-1, emilin. MAGPs 1 and 2. and LTBP-1 were present in glomeruli of mouse, rat, and human kidney, where they were located predominantly in the mesangial extracellular matrix underlying glomerular endotheliumand basement membrane. Several of these proteins, as well as elastin, werealso expressed in the renal vasculature. While elastin localized to the glomerular vascular pole in afferent and efferent arterioles extending to Bowman's capsule, it was not found in the glomerular capillary tuft. Cultured mesangial cells of rat, mouse, and human kidneys expressed mRNAs of fibrillin-1, emilin, MAGP-2, and elastin, and the respective proteins localized within and outside of mesangial cells, as shown by immunocytochemistry. mRNA expression of fibrillin-1, emilin, and elastin was strong in quiescent mesangial cells; their gene expression was further up-regulated by transforminggrowth factor-pr, while it was transiently reduced when cells were exposedto mitogenic 10% fetal calf serum and platelet-derived growth factor. Conclusions. These findings demonstrate that specific elastic fiber proteins are produced and secreted by mesangial cells. This process is regulated by growth factors; Their abundance in the extracellular matrix of the mesangium is in keeping with the concept that elastic fiber proteins contribute to the mechanical stability and elastic strength of the glomerular capillary tuft.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 10:55:40