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Titolo:
GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IS OVERPRODUCED BY KERATINOCYTES IN ATOPIC-DERMATITIS - IMPLICATIONS FOR SUSTAINED DENDRITIC CELL ACTIVATION IN THE SKIN
Autore:
PASTORE S; FANALESBELASIO E; ALBANESI C; CHINNI LM; GIANNETTI A; GIROLOMONI G;
Indirizzi:
IRCCS,IST DERMOPAT IMMACOLATA,IMMUNOL LAB,VIA MONTI CRETA 104 I-00167ROME ITALY IRCCS,IST DERMOPAT IMMACOLATA,DIV DERMATOL 8 I-00167 ROME ITALY UNIV MODENA,DEPT DERMATOL I-41100 MODENA ITALY
Titolo Testata:
The Journal of clinical investigation
fascicolo: 12, volume: 99, anno: 1997,
pagine: 3009 - 3017
SICI:
0021-9738(1997)99:12<3009:GCFIOB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; MESSENGER-RNA; GM-CSF; EXPRESSION; CYTOKINES; ASTHMA; GROWTH; INTERLEUKIN-4; PROLIFERATION; PERTURBATION;
Keywords:
INFLAMMATION; ATOPIC DERMATITIS; ANTIGEN-PRESENTING CELLS; CYTOKINES; EPITHELIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
S. Pastore et al., "GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IS OVERPRODUCED BY KERATINOCYTES IN ATOPIC-DERMATITIS - IMPLICATIONS FOR SUSTAINED DENDRITIC CELL ACTIVATION IN THE SKIN", The Journal of clinical investigation, 99(12), 1997, pp. 3009-3017

Abstract

Lesional skin of atopic dermatitis (AD) harbors high numbers of dendritic cells with enhanced stimulatory capacity for T lymphocytes. In this study, lesional AD skin was shown to stain heavily in both epidermal and dermal compartments for GM-CSF, a cytokine crucial to dendritic cell functions. Keratinocyte cultures established from uninvolved skinof AD patients exhibited markedly increased spontaneous and PMA-stimulated release of GM-CSF compared with keratinocytes from nonatopic controls. Correspondingly, keratinocytes from AD patients showed higher constitutive as well as PMA-induced GM-CSF gene expression. Larger amounts of GM-CSF were produced by AD keratinocytes, also in response to IL-1 alpha, but not after stimulation with LPS, lipoteichoic acid, or staphylococcal enterotoxin B. Hydrocortisone reduced GM-CSF gene expression and protein release in both atopic and control keratinocytes. Supernatants from atopic keratinocytes were able to strongly stimulate PBMC proliferation in a GM-CSF-dependent manner. Moreover, conditioned medium from PMA-treated AD keratinocytes, together with exogenous IL-4,could support phenotypical and functional maturation of peripheral blood precursors into dendritic cells. Enhanced production of GM-CSF by keratinocytes may contribute relevantly to the establishment and chronicity of AD lesions, in particular to the increased number, sustained activation, and enhanced antigen-presenting functions of dendritic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:58:50