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Titolo:
EXPRESSION OF THE BUMETANIDE-SENSITIVE NA-K-CL COTRANSPORTER BSC2 IS DIFFERENTIALLY REGULATED BY FLUID MECHANICAL AND INFLAMMATORY CYTOKINESTIMULI IN VASCULAR ENDOTHELIUM
Autore:
TOPPER JN; WASSERMAN SM; ANDERSON KR; CAI JX; FALB D; GIMBRONE MA;
Indirizzi:
BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV VASC RES,221 LONGWOOD AVE,LMRC 401 BOSTON MA 02115 BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV VASC RES BOSTON MA 02115 BRIGHAM & WOMENS HOSP,DEPT MED,DIV CARDIOVASC BOSTON MA 02115 HARVARD UNIV,SCH MED BOSTON MA 02115 MILLENNIUM PHARMACEUT CAMBRIDGE MA 02139
Titolo Testata:
The Journal of clinical investigation
fascicolo: 12, volume: 99, anno: 1997,
pagine: 2941 - 2949
SICI:
0021-9738(1997)99:12<2941:EOTBNC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; GLUTAMYL-TRANSFERASE TRANSPEPTIDASE; MOLECULAR-CLONING; GENE-EXPRESSION; MESSENGER-RNA; FUNCTIONAL EXPRESSION; ADHESION MOLECULE-1; INTERFERON-GAMMA; GROWTH-FACTOR; B CHAIN;
Keywords:
NA-K-CL COTRANSPORT; ENDOTHELIUM; CYTOKINES; SHEAR STRESS; INFLAMMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
J.N. Topper et al., "EXPRESSION OF THE BUMETANIDE-SENSITIVE NA-K-CL COTRANSPORTER BSC2 IS DIFFERENTIALLY REGULATED BY FLUID MECHANICAL AND INFLAMMATORY CYTOKINESTIMULI IN VASCULAR ENDOTHELIUM", The Journal of clinical investigation, 99(12), 1997, pp. 2941-2949

Abstract

In vascular endothelium, the electroneutral Na-K-Cl co-transport system is thought to function in the maintenance of a selective permeability barrier in certain vascular beds (e.g., brain), as well as in the preservation of endothelial homeostasis in the face of fluctuating osmotic conditions that may accompany certain pathophysiological conditions (e.g., diabetes mellitus). Here we demonstrate that the gene encoding the bumetanide-sensitive cotransporter BSC2, one of the two major isoforms of Na-K-Cl cotransporters present in mammalian cells, can be differentially regulated by inflammatory cytokines and fluid mechanical forces in cultured endothelium. Interleukin-1 beta and tumor necrosis factor-alpha significantly upregulate expression of BSC2 mRNA and protein in human umbilical vein endothelial cells, a response that is inhibited by pretreatment with interferon-gamma. Steady laminar fluid shear stress, at a physiologic magnitude (10 dyn/cm(2)), is also able to induce and maintain elevated expression of BSC2 in cultured human umbilical vein endothelial cells, while a comparable time-averaged magnitude of turbulent fluid shear stress is not. In vivo, BSC2 mRNA is upregulated after intraperitoneal administration of bacterial endotoxin (LPS) in murine lung and kidney, but not in cardiac tissue. These results provide the first experimental evidence that the BSC2 gene can be selectively regulated by different inflammatory cytokine and fluid mechanical stimuli in endothelium, and support a role for BSC2 in vascular homeostasis and inflammation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:23:26