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Titolo:
L-arginine protects and exacerbates ethanol-induced rat gastric mucosal injury
Autore:
Kalia, N; Bardhan, KD; Reed, MWR; Jacob, S; Brown, NJ;
Indirizzi:
Royal Hallamshire Hosp, Dept Surg & Anaesthet Sci, Sheffield S10 2JF, S Yorkshire, England Royal Hallamshire Hosp Sheffield S Yorkshire England S10 2JF ire, England
Titolo Testata:
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
fascicolo: 8, volume: 15, anno: 2000,
pagine: 915 - 924
SICI:
0815-9319(200008)15:8<915:LPAEER>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS NITRIC-OXIDE; DAMAGE; MICROCIRCULATION; STIMULATION; INHIBITION; CAPSAICIN; LEAKAGE;
Keywords:
gastric microcirculation; in vivo microscopy; L-arginine; macromolecular leakage; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kalia, N Royal Hallamshire Hosp, Dept Surg & Anaesthet Sci, K Floor,Glossop Rd, Sheffield S10 2JF, S Yorkshire, England Royal Hallamshire Hosp K Floor,Glossop Rd Sheffield S Yorkshire England S10 2JF
Citazione:
N. Kalia et al., "L-arginine protects and exacerbates ethanol-induced rat gastric mucosal injury", J GASTR HEP, 15(8), 2000, pp. 915-924

Abstract

Background and Aims: We have previously demonstrated that 60% ethanol (EtOH) increases macromolecular leakage and induces focal lesion formation in areas of permanent flow stasis within gastric mucosal vessels. Nitric oxide (NO) may prevent lesion formation by inhibiting leakage. This study used fluorescent in vivo microscopy to investigate (i) whether L-arginine (NO precursor) prevented EtOH induced injury and (ii) the mechanisms of protection. Methods: Experiments were carried out on anaesthetized rats (hypnorm/diazepam) receiving either intra-arterial fluorescein isothiocyanate-bovine serum albumin (0.2 mL/100 g), a marker for quantitating leakage, or Acridine red (0.1 mL/100 g) which labels leukocytes. Animals then received 100, 300 or500 mg/kg L-arginine (i.a.) followed by 60% EtOH or distilled water, topically applied to the gastric mucosa (n = 6 for each group). Vessel diameter,macromolecular leakage of labelled albumin from post capillary venules (PCV) and capillaries and leukocyte activity were quantitated using image analysis. Results: L-Arginine (100 mg/kg) did not increase vessel diameter or prevent EtOH-induced lesion formation and leakage. Both 300 mg/kg and 500 mg/kg alone induced significant and sustained increases in PCV diameter after 15 (P < 0.01) and 5 min (P < 0.001), respectively. Lesion formation was only prevented by 300 mg/kg L-arginine, whereas 500 mg/kg exacerbated haemorrhagiclesion formation over the entire exposed mucosa. Neither 300 mg/kg nor 500mg/kg L-arginine prevented leakage following EtOH. No leukocyte activity was observed following EtOH with or without L-arginine pretreatment. Conclusion: L-Arginine (300 mg/kg) prevented lesion formation. The mechanism of protection probably involved the increased blood flow in the dilated PCV and not the inhibition of leakage. The combined effects of EtOH and thepossible high NO levels exacerbate gastric mucosal damage despite the increases observed in vessel diameter. (C) 2000 Blackwell Science Asia Pry Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:26:52