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Titolo:
Differential regulation of trophic factor receptor mRNAs in spinal motoneurons after sciatic nerve transection and ventral root avulsion in the rat
Autore:
Hammarberg, H; Piehl, F; Risling, M; Cullheim, S;
Indirizzi:
Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden Karolinska InstStockholm Sweden S-17177 osci, S-17177 Stockholm, Sweden
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 4, volume: 426, anno: 2000,
pagine: 587 - 601
SICI:
0021-9967(20001030)426:4<587:DROTFR>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA INHIBITORY FACTOR; CILIARY NEUROTROPHIC FACTOR; MESSENGER-RNA EXPRESSION; GENE-RELATED PEPTIDE; INDUCED CELL-DEATH; RETROGRADE AXONAL-TRANSPORT; PROTEIN-TYROSINE KINASE; FACIAL MOTOR-NEURONS; MICE LACKING GDNF; MOLECULAR-CLONING;
Keywords:
nerve injury; regeneration; survival; in situ hybridization;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
118
Recensione:
Indirizzi per estratti:
Indirizzo: Hammarberg, H Karolinska Inst, Dept Neurosci, Nobels 12A, S-17177 Stockholm, Sweden Karolinska Inst Nobels 12A Stockholm Sweden S-17177 Sweden
Citazione:
H. Hammarberg et al., "Differential regulation of trophic factor receptor mRNAs in spinal motoneurons after sciatic nerve transection and ventral root avulsion in the rat", J COMP NEUR, 426(4), 2000, pp. 587-601

Abstract

After sciatic nerve lesion in the adult rat, motoneurons survive and regenerate, whereas the same lesion in the neonatal animal or an avulsion of ventral roots from the spinal cord in adults induces extensive cell death among lesioned motoneurons with limited or no axon regeneration. A number of substances with neurotrophic effects have been shown to increase survival of motoneurons in vivo and in vitro. Here we have used semiquantitative in situ hybridization histochemistry to detect the regulation in motoneurons of mRNAs for receptors to ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) 1-42 days after the described three types of axon injury. After all types of injury, themRNAs for GDNF receptors (GFR alpha-1 and c-RET) and the LIF receptor LIFRwere distinctly (up to 300%) up-regulated in motoneurons. The CNTF receptor CNTFR alpha mRNA displayed only small changes, whereas the mRNA for membrane glycoprotein 130 (gp130), which is a critical receptor component for LIF and CNTF transduction, was profoundly down-regulated in motoneurons afterventral root avulsion. The BDNF full-length receptor trkB mRNA was up-regulated acutely after adult sciatic nerve lesion, whereas after ventral root avulsion trkB was down-regulated. The NT-3 receptor trkC mRNA was strongly down-regulated after ventral root avulsion. The results demonstrate that removal of peripheral nerve tissue from proximally lesioned motor axons induces profound down-regulations of mRNAs for critical components of receptors for CNTF, LIF, and NT-3 in affected motoneurons, but GDNF receptor mRNAs are up-regulated in the same situation. These results should be considered inrelation to the extensive cell death among motoneurons after ventral root avulsion and should also be important for the design of therapeutical approaches in cases of motoneuron death. J. Comp. Neurol. 426:587-601, 2000. (C)2000 Wiley-Liss, Inc.

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Documento generato il 29/03/20 alle ore 12:10:20