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Titolo:
The role of cytotoxic T lymphocytes in corneal allograft rejection
Autore:
Hegde, S; Niederkorn, JY;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Ophthalmol, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Ctr, Dept Ophthalmol, Dallas, TX 75390 USA Univ Texas, SW Med Ctr, Grad Program Immunol, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Grad Program Immunol, Dallas, TX 75390 USA
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 11, volume: 41, anno: 2000,
pagine: 3341 - 3347
SICI:
0146-0404(200010)41:11<3341:TROCTL>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-CD4 MONOCLONAL-ANTIBODY; PENETRATING KERATOPLASTY; GRAFT-REJECTION; ORAL IMMUNIZATION; ENDOTHELIAL-CELLS; HLA ANTIGENS; RAT; TRANSPLANTATION; SURVIVAL; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Niederkorn, JY Univ Texas, SW Med Ctr, Dept Ophthalmol, 5323 Harry Hines Blvd, Dallas, TX75390 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75390 90 USA
Citazione:
S. Hegde e J.Y. Niederkorn, "The role of cytotoxic T lymphocytes in corneal allograft rejection", INV OPHTH V, 41(11), 2000, pp. 3341-3347

Abstract

PURPOSE. Immunologic rejection constitutes a major barrier to the success of allogeneic corneal transplants, but the specific mediators and mechanisms of graft rejection are poorly understood. Several studies have implicatedcytotoxic T-lymphocyte (CTL) responses, typically associated with CD8(+) Tcells, in promoting corneal graft rejection. This study sought to test thehypothesis that CTLs are essential in promoting corneal graft rejection. METHODS. BALB/c donor corneas were grafted orthotopically onto C57BL/6, perforin knockout, or CD8(+) T-cell knockout mice. The tempo and incidence ofgraft rejection were observed for each group. In separate experiments, donor-specific CTL and delayed-type hypersensitivity (DTH) responses were tested at the time of graft rejection by a standard chromium release assay and an ear swelling assay, respectively. RESULTS. Perforin knockout and CD8(+) T-cell knockout mice were as effective as wild-type C57BL/6 control mice in rejecting BALB/c donor corneas. Furthermore, animals in all three groups were found to develop robust donor-specific DTH, not CTL, responses at the time of graft rejection. Histopathologically, the rejected corneas from all three groups contained a predominantly mononuclear cellular infiltrate. CONCLUSIONS. This study rejects the hypothesis that CD8(+) CTLs are essential in promoting corneal graft rejection and instead further implicates donor-specific DTH reactions as the relevant immune response during graft failure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 23:10:54